Acrylamide gels
A Polyacrylamide Gel is a separation matrix used in electrophoresis of biomolecules, such as proteins or DNA fragments.
Acrylamide gels: A polymer gel used for electrophoresis of DNA or protein to measure their sizes (in daltons for proteins, or in base pairs for DNA). See "Gel Electrophoresis".
Used to make polyacrylamide gels for separation of macromolecules by electrophoresis.
1959 - introduction of acrylamide gels (Raymond and Weintraub); accurate control of parameters such as pore size and stability
1964 - disc gel electrophoresis (Ornstein and Davis) ...
Agarose and acrylamide gels are the media commonly used for electrophoresis of proteins and nucleic acids.
Endonuclease An enzyme that cleaves its nucleic acid substrate at internal sites in the nucleotide sequence.
by Levy Ulanovsky (Harvard University Biolabs) described a new technique for binding a bulky protein (streptavidin) to the end of single-stranded DNA; modifying DNA in this way facilitates separation of larger single strands on polyacrylamide gels.
An electric current is passed through a medium containing the mixture, and each kind of molecule travels through the medium at a different rate, depending on its electrical charge and size. Agarose and acrylamide gels are the media commonly used for ...
Separation is based on these differences; typically smaller fragments travel farther through the media (gel) in which separation is carried out. Agarose and acrylamide gels are the media commonly used for electrophoresis of proteins and nucleic ...
 See also: DNA, Electrophoresis, Sequence, Gel, Protein
  
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