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Affinity chromatography

Biology AerobicAffymetrix

Affinity Chromatography
The goal of affinity chromatography is to separate all the molecules of a particular specificity from the whole gamut of molecules in a mixture such as a blood serum.

 


Affinity chromatography is a chromatographic method of separating biochemical mixtures, based on a highly specific biologic interaction such as that between antigen and antibody, enzyme and substrate, or receptor and ligand.

Affinity chromatography is a biochemical separation method that combines size fractionation capability of gel permeation chromatography with the ability to design a stationary phase that reversibly binds to a known subset of molecules.

affinity chromatography A method for separating molecules by exploiting their ability to bind specifically to other molecules. There are several types of biological affinity chromatography.

Any protein that binds tightly to a specific sugar or sugars. Used in affinity chromatography to purify glycoproteins and as reagents for detecting glycoproteins.
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Affinity chromatography (← links)
Dynamite (← links)
Saturate (← links)
Ointment base (← links)
Percoll (← links)
Plombage (← links)
Thin-layer electrophoresis (← links)
Kety-schmidt method (← links) ...

See also: Protein, Chromatography, Proteins, Molecule, Trans