Alkaline Phosphatase

Alkaline phosphatase (ALP) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group...
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Alkaline phosphatase
- An enzyme that removes 5'-phosphate groups from the ends of DNA molecules, leaving 5'-hydoxyl groups
Allele ...

Various, eg, alkaline phosphatase
Cell attachment-recognition site
Various proteins involved in cell-cell (eg, sperm-oocyte), virus-cell, bacterium-cell, and hormone cell interactions ...

Several probe-enzyme conjugates, both direct oligonucleotide-alkaline phosphatase conjugates and indirect biotin-streptavidin-AP or digoxigenin-anti-digoxigenin-AP, are being tested.

The gene encoding alkaline phosphatase. Alkaline phosphatase is inactive unless it is exported outside of the cytoplasm where it becomes oxidized.

In order to detect the antibodies which have bound, anti-immunoglobulin antibodies coupled to a reporter group such as the enzyme alkaline phosphatase are added (e.g. goat anti-human IgG- alkaline phosphatase).

Removal of 5' phosphate from an RNA or DNA molecule, using alkaline phosphatase.
Plaque liftBlot taken from plaques (usually bacteriophage on a bacterial lawn) growing on an agar plate.

See also: Phosphatase, Trans, Enzyme, Protein, Action