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Amplification

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Amplification
PCR (in vitro DNA amplification)
Described as being to genes what Gutenberg's printing press was to the written word, ...
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The amplification of two or more target sequences in the same reaction.
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PCR (in vitro DNA amplification)
Described as being to genes what Gutenberg's printing press was to the written word, ...

Dr. Lawrence Brody, of the National Human Genome Research Institute's Genetic and Molecular Biology Branch, defines gene amplification.
related
terms:
cell, deoxyribonucleic acid (DNA), gene, oncogene ...

Amplification
As in PCR Amplification - exponential increase of the number of copies of a specific DNA sequence in vitro by using the polymerase chain reaction.

AMPLIFICATION - Refers to the production of additional copies of a chromosomal sequence, found either as intrachromosomal or extrachromosomal DNA. Also refers to the in vitro process in the polymerase chain reaction.

Amplification
An increase in the number of copies of a specific DNA fragment; can be in vivo or in vitro.
See also: cloning, polymerase chain reaction
Animal model ...

Amplifications (or gene duplications) leading to multiple copies of all chromosomal regions, increasing the dosage of the genes located within them.
Deletions of large chromosomal regions, leading to loss of the genes within those regions.

Amplification of RNAi
In C. elegans, plants, and Neurospora, the introduction of a few molecules of dsRNA has a potent and long-lasting effect.

amplification 1. Treatment (e.g., use of chloramphenicol) designed to increase the proportion of plasmid DNA relative to that of bacterial (host) DNA.
2. Replication of a gene library in bulk.
3. Duplication of gene(s) within a chromosomal segment.

Gene amplification
Multiple copies of a single gene within a cell.
Gene conversion
An genetic event that produces abnormal segregations by non-reciprocal recombination.

Gene amplification
- The increase, within a cell, of the number of copies of a given gene.
Gene bank ...

gene amplification
The selective synthesis of DNA, which results in multiple copies of a single gene, thereby enhancing expression.
gene cloning ...

Gene amplification: The process whereby genes or a sequence of DNA in the genome is greatly increased in number of copies.
Gene cloning: The technique of making many copies of a gene.

Gene Amplification
In Drosophila (fruit flies), the chorion (eggshell) gene is copied many times in certain cells of the oviduct. These cells make large quantities of the protein needed to surround the egg.

Gene amplification. The presence of multiple genes. Amplification is one mechanism through which proto-oncogenes are activated in malignant cells.

Signal amplification
A principle of signal transduction is the signal amplification. The binding of one or a few neurotransmitter molecules can enable the entry of millions of ions.

New DNA amplification technologies developed during the 1990s, such as the polymerase chain reaction (PCR), made obtaining sufficient quantities of DNA much easier.

Directed amplification of minisatellite region DNA (DAMD)
A technique for detecting polymorphisms using VNTR core sequences as primers for PCR.
Related Terms:
Polymorphism
Difference in DNA sequence among individuals.

Refers to amplification of specific alleles, or DNA sequence variants, at the same locus.

(Science: physics) (Amplification) increase in a signal transmitted from one point to another through an amplifier.
Please contribute to this project, if you have more information about this term feel free to edit this page ...

(See Dominant gene, Fusion gene, Gene amplification, Gene expression, Gene flow, Gene pool, Gene splicing, Gene translocation, Recessive gene, Regulatory gene.) Gene amplification. The presence of multiple genes.

Amplification -- any process by which specific DNA sequences are replicated disproportionately greater than their representation in the parent molecules. Aneuploidy -- state of having variant chromosome number (too many or too few). (i.e.

PCR (Polymerase Chain Reaction) A repetitive procedure that results in a geometric amplification of a specific DNA sequence.

PCR amplification is possible from complex sequence background e.g. a short sequence from an entire chromosome, and from impure DNA. The technique is widely used in many applications.

Expression of cathepsin B is regulated at many different levels, from gene amplification, use of alternative promoters, increased transcription and alternative splicing, to increased stability and translatability of transcripts.

The technology utilizes DNA fragments that are ligated to complementary adaptor oligonucleotide and subsequent rounds of PCR amplification using primers complementary to the adaptor sequences.

Branched DNA (bDNA) is a signal amplification nucleic acid probe assay for the direct quantitation of viral RNA in serum or plasma. One end of bDNA binds to a specific target and the other end has many branches of DNA.

(fluorescence in situ hybridization): One of the more modern methods in cytogenetics, which uses fluorescence-labelled chromosome-specific DNA, probes to detect translocations, inversions, deletions, amplifications and other structural or numerical ...

polymerase chain reaction - powerful DNA synthesis and amplification technique allowing the amplification of a specific sequence from among many others; thoeoretically PCR can be used to detect as little as one specific DNA molecule.

Levers amplify or increase the force or velocity of motion. The amount of amplification depends on the length of the lever. There are three types of skeletal system, all interact with muscles using the lever.

See also: DNA, Sequence, PCR, Genome, Trans