Autoradiography

Autoradiography
If the mixture contains molecules that have been labeled with a radioactive tracer, these can be located by placing the chromatogram next to a sheet of X-ray film.

Autoradiography: A technique that uses X-ray film to visualize radioactively labeled molecules or fragments of molecules; used in analyzing length and number of DNA fragments after they are separated by gel electrophoresis.

Autoradiography A technique which reveals the position of radioactive isotope (such as 35S, 32P or 33P) within an experimental result.

autoradiography - photographic process used to detect location of radioactive material.
axoneme - the motor section of the flagellum which is a construction of microtubules emanating from the centriole at the base of the flagellum.

Autoradiography
A photographic image produced exposing photographic film to a radiolabeled molecule (e.g. DNA, RNA, or protein). The emissions from the radioactive label expose the photographic film.
Autoregulation
See autogenous regulation.

Autoradiography A means of detecting radioactive molecules immobilized in a separation medium such as polyacrylamide; the radioactivity of the molecules will blacken x-ray film.

Autoradiography is a process in which film is used to show the area of the vector where the probe has attached. This area is the gene in question.
Obtaining Probes ...

SDS-PAGE autoradiography
Gel electrophoresis is a group of techniques used by scientists to separate molecules based on physical characteristics such as size, shape, or isoelectric point.

SDS-PAGE autoradiography - The indicated proteins are present in different concentrations in the two samples.

Stage 5 - Autoradiography
Radioactive solution is washed off and an X-Ray plate is placed over the membrane ...

Detection of RFLPs via autoradiography
The locations of radioactive probe hybridization on the Southern blot are detected by autoradiography.

A technique of blotting cloned DNA without prior restriction digestion and electrophoresis (See Southern blotting). Autoradiography reveals dots indicating probe hybridization.
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...

A technique for amplifying anonymous stretches of DNA(single random primers of 16-20 bases) using PCR. Products are often analysed on a polyacrylamide gel and detected by autoradiography. Related to RAPD.
Randomly amplified polymorphic DNA (RAPD) ...

The extent of labeling (as determined by autoradiography and densitometry) is proportional to the concentration of the target molecule in the sample. Standards provide a means of calibrating the results.

DNA is directly labelled at the restiriction sites and two-dimensionall electrophoresis is employed to resolve and identify these landmarks. The procedure gives a 2-D pattern with thousands of scattered spots of landmarks after autoradiography.

resolved DNA segments from an agarose gel to a nitrocellulose filter paper sheet via capillary action; the DNA segment of interest is probed with a radioactive, complementary nucleic acid, and its position is determined by autoradiography.

See also: Autoradiograph, DNA, Molecule, Electrophoresis, Trans