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Bacteriophage lambda

Biology BacteriophageBacteriophages

Bacteriophage Lambda binds to the target E. coli cell, the J protein in the tail tip interacting with the lamB gene product of E. coli, a porin molecule which is part of the maltose operon.

 


Bacteriophage lambda: A virus which infects E. coli , and which is often used in molecular genetics experiments as a vector, or cloning vehicle.

the DNA of bacteriophage lambda, a virus that infects E. coli, and which serves here as the vector for
3 genetic elements from the lac operon of E. coli
the lacI gene
the operator of the operon
the beta-galactosidase (lacZ) gene ...

Participants used restriction enzymes to cut DNA from the bacteriophage lambda and analyzed the resulting DNA fragments by agarose gel electrophoresis.

If an entire molecule of DNA from the virus "bacteriophage lambda" were shown at this scale, the image would be 970 meters high. For the bacterium Escherichia coli, the image would be 80 kilometers long.

COSMID - A genetically-engineered plasmid containing bacteriophage lambda packaging signals and potentially very large pieces of inserted foreign DNA (up to 50 kb) which can be replicated in bacterial cells.

lambda Any type of cloning vector (cosmids) containing cos sites necessary for propagation in bacteriophage lambda. Cosmid clones can be used to clone DNA fragments of up to 15 kb.
library A collection of clones in any type of vector.

- A hybrid vector made up of plasmid sequences and the cohesive ends of the bacteriophage lambda
Cotton ...

Bacteriophage A virus which infects bacteria. Modified bacteriophages are used as cloning vectors. Two which are commonly encountered are bacteriophage lambda and bacteriophage M13.

See also: Bacteriophage, Phage, DNA, Lambda, Bacteria