Biotin
(Science: biochemistry) a prosthetic group for carboxylase enzymes.
Biotin
A molecule that can be incorporated into dUTP and used as a non-radioactive label for a DNA probe.
Biotin a B vitamin related to vitamin B2 and formerly called vitamin H
(bios = life)
Bivalent a pair of homologous chromosomes during meiosis‚ also known as a tetrad
(bi = two; valent = strength) ...
Biotin A vitamin that plays a role in carboxylation and decarboxylation reactions.
Blymphocyte Precursors to plasma cells, which are antibody-secreting cells.
Biotinylation
labelling a probe with congugated biotin, whose high affinity for avidin or anti-biotin antibody is exploited in immunoassays ...
Biotinylation of antibodies ... Conjugation of monoclonal antibodies to fluorescent dyes - an in-depth guide to ... Immunoprecipitation with soluble antibodies ...
Full article ...
Biotin has an extraordinarily high affinity for streptavidin with a reported dissociation constant of ~10-15. This very strong binding affinity has made the biotin-streptavidin system very attractive for a multitude of in vitro labeling applications.
The label may be radioactive (32P), biotin or digoxygenin (DIG).
PromoterA short base sequence which is positioned close to the 5" end of a gene and acts as a recognition and binding site for the RNA polymerase complex prior to transcription of ...
The "male" lacks functional genes needed to synthesize the vitamin biotin and the amino acid methionine (Bio−, Met−) so these must be added to its culture medium.
For example, biotin is part of enzymes involved in making fatty acids. Vitamins also act as coenzymes to carry chemical groups between enzymes.
A probe can be radiolabeled, or tagged with another functional group such as biotin. A probe can be cloned DNA, or might be a synthetic DNA strand.
coenzyme An organic nonprotein molecule, frequently a phosphorylated derivative of a water-soluble vitamin, that binds with the protein molecule (apoenzyme) to form the active enzyme (holoenzyme). Examples include biotin, NAD+, and coenzyme A.
 See also: DNA, Protein, Proteins, Enzyme, Molecule
  
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