Blunt ends
Digestions of double-stranded DNA by many restriction enzymes (e.g. EcoR V) generate ends without any single-stranded sequences. Such ends are called blunt ends.
Related ...
Sticky end/blunt end
In biology, sticky end and blunt end are the two possible configurations resulting from the breaking of double-stranded DNA.
[edit] Blunt ends
The simplest DNA end of a double stranded molecule is called a blunt end. In a blunt-ended molecule both strands terminate in a base pair.
Blunt ends
- DNA termini without overhanging 3' or 5' ends. Also knows as flush ends ...
For sequencing purposes at Los Alamos, we have previously used blunt end ligation of inserts into the HincII site of pUC-18. In addition, we have also used the double adaptor approach described by Andersson et al.
In the cervical and upper thoracic regions they appear as a comma-shaped fasciculus in the lateral part of the fasciculus cuneatus, the blunt end of the comma being directed toward the posterior gray commissure; ...
This produces either "sticky ends" or "blunt ends"
DNA ligase can be used to re-join the ends ...
In some needles(as for sewing machines) the eye is at the pointed end, but in ordinary needles it is at the blunt end.
2. See magnetic needle, under Magnetic.
Some restriction enzymes generate blunt ends, cutting across both strands of DNA. Others generate a staggered cut, producing "sticky ends.
Blunt endEnd of a DNA fragment produced by a restriction enzyme which cuts both strands of DNA at the same point, leaving no single-stranded sections. DNA with this type of end is more difficult to ligate ...
 See also: DNA, Enzyme, Strand, Molecule, Sequence
  
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