Blunt ends
Digestions of double-stranded DNA by many restriction enzymes (e.g. EcoR V) generate ends without any single-stranded sequences. Such ends are called blunt ends.
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[edit] Blunt ends
The simplest DNA end of a double stranded molecule is called a blunt end. In a blunt-ended molecule both strands terminate in a base pair.
Blunt ends
- DNA termini without overhanging 3' or 5' ends. Also knows as flush ends ...
of total genomic mammalian DNA. The basic method consists of 1) treatment of the genomic DNA with sequence specific cleavage agents, 2) primer extension with a gene specific oligonucleotide 3) ligation of an oligonucleotide to the blunt ends, 4) ...
The two fragments may then be covalently bonded by DNA ligase. Blunt ends may be ligated, but the reaction is significantly slower. These terms are most commonly used to describe the product of a restriction enzyme digestion of DNA.
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This produces either "sticky ends" or "blunt ends"
DNA ligase can be used to re-join the ends ...
Some restriction enzymes generate blunt ends, cutting across both strands of DNA. Others generate a staggered cut, producing "sticky ends.
 See also: DNA, Enzyme, Blunt End, Strand, Biology
  
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