Chromosome walking is a technique to clone a gene (e.g., a disease gene) from its known closest markers. The closest linked marker (e.g., EST or a known gene) to the gene is used to probe a genomic library.
Chromosome walking is a method in genetics for identifying and sequencing long parts of a DNA strand, e.g., a chromosome.
A technique used to identify a series of overlapping restriction fragments, often to determine the relative positions of genes on large chromosomes.
Chromosome walking. Working from a flanking DNA marker, overlapping clones are successively identified that span a chromosomal region of interest. (See Chromosome.) ...
Method involving hybridizing a primer of known sequence to a clone from an unordered genomic library and synthesizing a short complementary strand (called walking along a chromosome).
Chromosome walking: A technique for cloning everything in the genome around a known piece of DNA (the starting probe).
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Starting at DNA clones from both ends of this region, the researchers used chromosome walking to clone all of the interval; several candidate genes were identified within the region but rejected as the cause of CF.
See also: DNA, Chromosome, Chromosome walking, Strand, Sequence