 See also: Chromosome walking, Chromosome, DNA, Genome, Sequence
Chromosome walking
Chromosome walking is a technique to clone a gene (e.g., a disease gene) from its known closest markers. The closest linked marker (e.g., EST or a known gene) to the gene is used to probe a genomic library.
Chromosome walking is a method in genetics for identifying and sequencing long parts of a DNA strand, e.g., a chromosome.
Cloning a Disease Gene by Chromosome Walking.
After a marker is linked to within 1 cM of a disease gene, chromosome walking can be used to clone the disease gene itself.
Chromosome walking
A technique used to identify a series of overlapping restriction fragments, often to determine the relative positions of genes on large chromosomes.
Chromosome walking A method of making a detailed map of a chromosome by isolating successive overlapping clones. Usually each clone is used as a probe to isolate the next.
Clone ...
Chromosome walking. Working from a flanking DNA marker, overlapping clones are successively identified that span a chromosomal region of interest. (See Chromosome.) ...
Chromosome walking
Method involving hybridizing a primer of known sequence to a clone from an unordered genomic library and synthesizing a short complementary strand (called walking along a chromosome).
CHROMOSOME WALKING - The sequential isolation of clones carrying overlapping sequences of DNA which span large regions of a chromosome. Overlapping regions of clones can be identified by hybridization.
Chromosome walking: A technique for cloning everything in the genome around a known piece of DNA (the starting probe).
(Redirected from Chromosome walking)
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Starting at DNA clones from both ends of this region, the researchers used chromosome walking to clone all of the interval; several candidate genes were identified within the region but rejected as the cause of CF.
See also: Chromosome walking, Chromosome, DNA, Genome, Sequence
  
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