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Chromosome walk

Biology  Chromosome pairing  Chromosome walking

Chromosome walking
Chromosome walking is a technique to clone a gene (e.g., a disease gene) from its known closest markers. The closest linked marker (e.g., EST or a known gene) to the gene is used to probe a genomic library.

Chromosome walking is a method in genetics for identifying and sequencing long parts of a DNA strand, e.g., a chromosome.

Chromosome walking
A technique used to identify a series of overlapping restriction fragments, often to determine the relative positions of genes on large chromosomes.

Chromosome walking
Method involving hybridizing a primer of known sequence to a clone from an unordered genomic library and synthesizing a short complementary strand (called walking along a chromosome).

Chromosome walking: A technique for cloning everything in the genome around a known piece of DNA (the starting probe). You screen a genomic library for all clones hybridizing with the probe, and then figure out which one extends furthest into the surrounding DNA.

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(See ~ing.) ~ing. Working from a flanking DNA marker, overlapping clones are successively identified that span a chromosomal region of interest. (See Chromosome.) Cistron. A DNA sequence that codes for a specific polypeptide; a gene. See DNA, Gene. Clone.

Starting at DNA clones from both ends of this region, the researchers used ~ing to clone all of the interval; several candidate genes were identified within the region but rejected as the cause of CF.

Acevedo-Garcia J, Collins NC, Ahmadinejad N, Ma L, Houben A, Bednarek P, Benjdia M, Freialdenhoven A, Altmuller J, Nurnberg P, Reinhardt R, Schulze-Lefert P, Panstruga R: Fine mapping and ~ing towards the Ror1 locus in barley (Hordeum vulgare L.). ...

See also: See also: What is the meaning of Chromosome, Chromosome walking, DNA, Sequence, Genome?

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