Chromosome walking

Chromosome walking
Chromosome walking is a technique to clone a gene (e.g., a disease gene) from its known closest markers. The closest linked marker (e.g., EST or a known gene) to the gene is used to probe a genomic library.

Chromosome walking is a method in genetics for identifying and sequencing long parts of a DNA strand, e.g., a chromosome.

Cloning a Disease Gene by Chromosome Walking.
After a marker is linked to within 1 cM of a disease gene, chromosome walking can be used to clone the disease gene itself.

Chromosome walking
A technique used to identify a series of overlapping restriction fragments, often to determine the relative positions of genes on large chromosomes.

Chromosome walking A method of making a detailed map of a chromosome by isolating successive overlapping clones. Usually each clone is used as a probe to isolate the next.
Clone ...

Chromosome walking. Working from a flanking DNA marker, overlapping clones are successively identified that span a chromosomal region of interest. (See Chromosome.) ...

Chromosome walking A technique for analyzing long stretches of DNA by sequential subcloning and rescreening of overlapping segments.

Chromosome walking
Method involving hybridizing a primer of known sequence to a clone from an unordered genomic library and synthesizing a short complementary strand (called walking along a chromosome).

CHROMOSOME WALKING - The sequential isolation of clones carrying overlapping sequences of DNA which span large regions of a chromosome. Overlapping regions of clones can be identified by hybridization.

Chromosome walking: A technique for cloning everything in the genome around a known piece of DNA (the starting probe).

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See also: Chromosome, Chromosome walk, DNA, Sequence, Clone