Constructing Clones for Sequencing.
Cloned DNA molecules must be made progressively smaller and the fragments subcloned into new vectors to obtain fragments small enough for use with current sequencing technology.
Clones: A group of cells derived from a single ancestor.
Cloning: The process of asexually producing a group of cells (clones), all genetically identical, from a single ancestor.
cDNA clones are PCR amplified and the PCR products are printed on to slides with micropins. The clones are verified by sequencing. cDNA array is hybridized with Cy-dye labeled sample and identical control for the same set of experiments.
Such clones are not strictly identical since the somatic cells may contain mutations in their nuclear DNA.
mutant clones in the posterior compartment or far from the A/P boundary develop normally (Chen, 1996)
Two activated Smo mutants, SmoM1 and SmoM2, were isolated from human basal cell carcinomas and are resistant to Ptc1 inhibition.
All those clones that continue to grow on ampicillin but fail to grow on kanamycin (here, clones 2, 5, and 8) have been transformed with a piece of human DNA.
Some recombinant DNA products being used in human therapy ...
Overlapping clones
See genomic library.
Related Terms:
Genomic library
A collection of clones made from a set of randomly generated overlapping DNA fragments representing the entire genome of an organism. Compare library, arrayed library.
clone seq Clones that are mapped onto the genome sequence include annotation units (e.g. BACS used to assemble the genome sequence), full length cDNAs and expressed sequence tags.
A collection of clones made from a set of randomly generated overlapping DNA fragments that represent the entire genome of an organism.
See also: library, arrayed library
Genomic sequence
See: DNA ...
Contig
Group of clones representing overlapping regions of a genome.
Related Terms:
Clone bank
See genomic library.
- A collection of clones prepared from the mRNA of a given cell or tissue type, representing the genetic information expressed by such cells
Cell
- The smallest structural unit of living organisms that is able to grow and reproduce independently.
Working from a flanking DNA marker, overlapping clones are successively identified that span a chromosomal region of interest. (See Chromosome.) Cistron. A DNA sequence that codes for a specific polypeptide; a gene. See DNA, Gene. Clone.
Library A collection of clones or DNA fragments which contains all of the sequences present in the source.
Many horticultural varieties of plants are clones, having been derived from a single individual, multiplied by some process other than sexual reproduction.
Histochemical, immunohistochemical and functional analysis of the resulting cybrids all showed a pattern in the PD clones indicative of a mtDNA mutation.
By repeating this process with the thousands of clones that span each chromosome, researchers can determine the sequences of all the larger clones.
You screen a genomic library for all clones hybridizing with the probe, and then figure out which one extends furthest into the surrounding DNA.
Monoclonal antibodies come from clones of B cells that produce a single antibody of known specificity. B cells will nor normally divide in the absence of antibody.
CHROMOSOME WALKING - The sequential isolation of clones carrying overlapping sequences of DNA which span large regions of a chromosome. Overlapping regions of clones can be identified by hybridization.
library. In molecular biology, a set of clones containing recombinant DNA. Obtained from and representing the genome of the organism.
lichen An organism composed of a symbiotic association of an ascomycete fungus with algal or cyanobacterial cells.
(Science: biology) a group of identical cells (clones) derived from a single parent cell.
(Science: cell culture) a clone of bacterial cells on a solid medium that is visible to the naked eye.
Library A large bank of clones (which may be either genomic or cDNA). The depth of a genomic library is the number of times which any single copy sequence can be expected to be recovered from it.
Genomic library
A collection of clones that includes essentially all the genes of a particular organism.
Chromosome walking. Working from a flanking DNA marker, overlapping clones are successively identified that span a chromosomal region of interest. (See Chromosome.) ...
Cloning: The process of asexually producing a group of cells (clones), all genetically identical, from a single ancestor.
clone -- An identical copy of an organism. Most plants, fungi, algae, and many other organisms naturally reproduce by making clones of themselves as a form of asexual reproduction.
cnidocyst -- The "stinging cell" of a cnidarian.
Offspring have some genes from each of two different parents and therefore are not identical clones of their parents.
Since bacteria reproduce asexually all of its descendents are clones. Since reproduction is not perfect mutations happen. The experimenter can set the environment so that mutations for a particular attribute are selected.
recombinant DNA technology A series of techniques in which DNA fragments are linked to self-replicating forms of DNA to create recombinant DNA molecules. These molecules in turn are replicated in a host cell to create clones of the inserted ...
normally kill all other members of that clone (colony) it comes in contact with. Evolution requires genetic variation on which to operate. How then can bacteria increase their genetic variation if their typical mode of reproduction produces clones?
 See also: Clone, Human, DNA, Genome, Sequence
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