Cloning vector

Cloning Vector. Despite the advantages of certain lambda vectors to preferentially clone longer cDNAs, plasmids are considered advantageous given the ease of subsequent manipulation, sequence generation, ...

Cloning Vector: DNA molecule originating from a virus, a plasmid, or the cell of a higher organism into which another DNA fragment of appropriate size can be integrated without loss of the vectors capacity for self- replication; ...

Cloning vector A DNA molecule that is capable of replication in a suitable host cell, that has suitable site(s) for the insertion of DNA fragments by recombinant DNA techniques, ...

cloning vector - intentionally designed artificial DNA construct used by molecular biologists to amplify selected pieces of DNA inserted into the construct; examples include plasmid, phage, phagemid, cosmid, fosmid, ...

cloning vector An agent used to transfer DNA in genetic engineering, such as a plasmid that moves recombinant DNA from a test tube back into a cell, or a virus that transfers recombinant DNA by infection. closed circulatory system ...

cloning vector A small, self-replicating DNA molecule - usually a plasmid or viral DNA chromosome - into which foreign DNA is inserted in the process of cloning genes or other DNA sequences of interest.

BAC is a cloning vector propagated as a mini-chromosome in a bacteria host. Inserts of 100-300 kb can be cloned in BAC vectors. Related Vector ...

See cloning vector. Related Terms: Cloning vector DNA molecule originating from a virus, a plasmid, ...

Vector See: cloning vector (ORNL) Virus A noncellular biological entity that can reproduce only within a host cell. Viruses consist of nucleic acid covered by protein; some animal viruses are also surrounded by membrane.

Cosmid A type of cloning vector which is based on a plasmid but which contains one copy (or sometimes two copies) of the lambda cohesive end.

lambda Any type of cloning vector (cosmids) containing cos sites necessary for propagation in bacteriophage lambda. Cosmid clones can be used to clone DNA fragments of up to 15 kb. library A collection of clones in any type of vector.

To increase the number of copies of a DNA sequence, in vivo by inserting into a cloning vector that replicates within a host cell, or in vitro by polymerase chain reaction (PCR). Anaerobe. An organism that grows in the absence of oxygen. See Aerobe.

Cloned DNA -- any DNA fragment that passively replicates in the host organism after it has been joined to a cloning vector. Codon -- a sequence of three nucleotides in mRNA that specifies an amino acid. Consanguinity -- genetic relationship.

A number of artificially constructed plasmids are used as cloning vectors. Polygenic disorders Genetic disorders resulting from the combined action of alleles of more than one gene (e.g., heart disease, diabetes, and some cancers).

Artificially constructed cloning vector containing the cos gene of phage lambda.

Modern cloning vectors include selectable antibiotic resistance markers, which allow only cells in which the vector has been transfected, to grow.

Phages play an important role in molecular biology as cloning vectors to insert DNA into bacteria.

BAC- Bacterial Artificial Chromosome - a cloning vector capable of carrying between 100 and 300 kilobases of target sequence. They are propagated as a mini-chromosome in a bacterial host.

To do this, a region on the chromosome is identified and DNA homologous to that region is engineered into a cloning vector.

A short DNA sequence containing several re-striction enzyme recognition sites that is contained in cloning vectors. A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...

See also: Cloning, Vector, DNA, Sequence, Chromosome