Cohesive ends
Single-stranded complementary sequences at two ends of a DNA molecule (examples include the ends of the linear phage lambda chromosome, and the ends resulting from digestion of DNA with many restriction endonucleases).
Cohesive end: Also known as sticky end. Overhanging ends of a double-stranded DNA molecule that are capable of hybridizing with complementary ends.
Cohesive ends In double-stranded DNA molecules, complementary single-stranded ends produced by staggered cuts.
Cohesive ends
- Those ends (termini) of DNA molecules that have short somplementary sequences that can stick together to join two DNA molecules. Often generated by restriction enzymes.
Competent ...
Cosmids -- plasmid vectors designed for cloning large fragments of eukaryotic DNA; the vector is a plasmid into which phage lambda cohesive end sites have been inserted. CpG islands -- areas of multiple CG repeats in DNA.
If DNA which is to be annealed to this is tailed with the complementary base, mutually cohesive ends are produced. The tailed molecules are unable to circularise because their ends are not cohesive. Sometimes used for insertion of DNA into vectors.
Longer overhangs are called cohesive ends or sticky ends. They are most often created by restriction endonucleases when they cut DNA.
Cosmid A type of cloning vector which is based on a plasmid but which contains one copy (or sometimes two copies) of the lambda cohesive end.
 See also: Enzyme, Nucleotide, Base, Molecule, Complement
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