The restoration of activity or partial activity (complementation) to an enzyme made of identical subunits (polypeptides encoded by one gene) in a heterozygote of two different mutant alleles of that gene.
As we saw above, rapid lysis (r) mutants were found that mapped to three different regions of the T4 genome: rI, rII, and rIII.
This meant that ...
Complementation. When two mutations are combined in an organism and the phenotype is wild type, the mutations are said to complement each other.
The ability of a gene to produce a functional gene product which compensates for the mutant phenotype caused by a mutation in another gene.
In genetics, the restoration of a wild-type function (e.g., ability to grow on galactose) in diploid heterozygotes generated from haploids, each of which carries a mutation in a different gene whose encoded protein is required for the same biochemical pathway.
~: The production of a wildtype phenotype when two recessive mutations from different genes are brought together.
The process by which two recessive mutants can supply each other's deficiency, such that a heterokaryon or diploid derived fron them and having the trans (repulsion) configuration is phenotypically normal (wild-type) or nearly so.
A mating test to determine whether two different recessive mutations (a1;a2) on opposite chromosomes (trans, a1+/+a2) of a diploid or partial diploid will not complement (ie have a mutant phenotype) each other; but the same two recessive mutations on the same chromosome (cis, ...
- Process by which genes on different DNA molecules interact. Usually a protein product is involved, as this is a diffusible molecule that can exert its effect away from the DNA itself.
Finally, CP ~ was explored between TMV and the heterologous Barley stripe mosaic virus (BSMV), a rod-shaped virus that exhibits CP sequence divergence to TMV and ToMV (Figure 5B). Although barley, Hordeum vulgare cv.
Protein-fragment ~ Assay (PCA) Any of a family of protein-protein interaction assays in which a bait protein is expressed as fusion to one of either N- or C-terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C-terminal ...
~ — The restoration of normal phenotype by gene replacement. The replaced gene can either be an intact copy of a defective gene (direct replacement), or a gene with function that can compensate for the defective gene's aberrant function.
A ~ group consisting of seven recessive lethal mutations was mapped to the Madm genomic locus (Materials and methods).
Synthetic lethality or unlinked non-~ - two mutations fail to complement and yet do not map to the same locus.
% recombination; mappable and unique; satisfy ~ or cis-trans test
A band or knob on a stained chromosome (insertions, deletions, translations) ...
AD, AR, XD, XR, CD: Patterns of Mendelian inheritance
Allelic and locus heterogeneity (~ tests)
Variable expressivity and incomplete penetrance
Quantitative Genetics ...
See also: What is the meaning of Complement, Trans, Express, Mutation, Protein?