Concatemer. A DNA segment composed of repeated sequences linked end to end. Conjugation. The joining of two bacteria cells when genetic material is transferred from one bacterium to another.
Notably, this doesn't release single copies of the phage genome, but rather one long molecule with many copies of the genome: a concatemer. These concatemers are cleaved at their cos sites as they are packaged.
CCS has been applied to the joining of multiple independent cDNA molecules to form long concatemers, and then generation of shotgun libraries.
See also: Clone, Gene, Chromosome, Membrane, Polymerase
|