Loss of natural configuration (of a molecule) through heat or other treatment. Fully denatured DNA is single-stranded.
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Denature. To induce structural alterations that disrupt the biological activity of a molecule.
Denature the process whereby a protein looses its native conformation
(de = from‚ down‚ out; nata = born‚ produced)
Dendrite the "incoming" end of a neuron
(dendro = tree) ...
[edit] Denatured alcohol
Main article: Denatured alcohol
Pure ethanol and alcoholic beverages are heavily taxed. Ethanol has many applications that do not involve human consumption.
Denature
To provoke structural changes in a molecule which disrupt its biological activity. In DNA it refers to the separation of the two component strands caused by the breaking of the hydrogen bonds.
denatured enzymes lose their catalytic power
denatured antibodies can no longer bind antigen
A mutation in the gene encoding a protein is a frequent cause of altered tertiary structure.
denatured DNA will not reanneal after it is diluted
The Biology Project
University of Arizona
Thursday, October 24, 1996
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denature To change the configuration of a protein molecule such that it loses specificity and no longer functions as an enzyme.
dendrite Any of nerve cell processes that conduct impulses toward the cell body.
Prions can be denatured by subjecting them to a temperatures of 134 degrees Celsius for 18 minutes in a pressurised steam autoclave.[32] Ozone sterilization is currently being studied as a potential method for prion deactivation.
denatured
Denaturing gradient gel electrophoresis (DGGE)
Denaturing high-performance liquid chromatography (DHPLC)
dendrites
Denhardt's solution
denominator element
density
density-gradient centrifugation
dentate nucleus ...
An in situ methodology employing solution conditions has been developed for binding oligodeoxyribonucleotide 'third-strands' to chromosomal DNA targets in non-denatured protein-depleted metaphase spreads and interphase nuclei.
High temperatures and extreme acid base changes denature (inactivate) them, they lose their specific shape and pockets and can no longer do their job. Most enzymes work best at neutral pH such as that found in the cytosol.
After electrophoresis, the DNA in the gel is denatured by soaking the gel in an alkaline solution. Then a nitrocellulose or nylon membrane is layed on top of the agarose gel and absorbant paper is layered on top of the membrane.
At the temperature DNA denatures (strands separate) proteins from most organisms (like DNA polymerases) also come apart. This presents a problem.
Caution: do not allow temperature to exceed 60 degrees because this may denature DNA.
4. Dissolve 3g meat tenderizer in the wheat germ solution and add 10ml baking soda solution. Maintain the temperature at 55 degrees for 10 minutes.
5.
C0t 1 DNA Eukaryotic DNA which has been denatured and allowed to reanneal to a C0t value of 1. The double stranded component is then purified from the single stranded component and is supplied commercially.
The cells are lysed and the DNA is denatured by treating it with an alkaline solution. When the probe is mixed with the denatured DNA, it will form a double-helix in the area where the gene has complimentary bases.
Tm - The midpoint of the temperature range over which DNA is melted or denatured by heat; the temperature at which a duplex nucleic acid molecule is 50% melted into single strands, ...
Changes in pH will also denature the enzyme by changing the shape of the enzyme. Enzymes are also adapted to operate at a specific pH or pH range.
Random primed synthesis: If you have a DNA clone and you want to produce radioactive copies of it, one way is to denature it (separate the strands), then hybridize to that template a mixture of all possible 6-mer oligonucleotides.
In situ hybridization A technique in which cells are immobilized and their DNA is denatured and then hybridized to radioactive RNA probes; these hybrids are then detected by autoradiography.
Some archaea produce unusually high concentrations of thermoprotective proteins (heat shock proteins), which are found in all cells. These proteins help refold partially denatured proteins.
 See also: DNA, Protein, Proteins, Molecule, Sequence
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