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Dideoxy Sequencing

Biology DicotDifferentiation

Dideoxy sequencing (also called chain- termination or Sanger method) uses an enzymatic procedure to synthesize DNA chains of varying lengths, stopping DNA replication at one of the four bases and then determining the resulting fragment lengths.

 


Such fragments are too long to be sequenced in a single sequence read using the cyclic dideoxy terminator method (also known as PCR based dideoxy sequencing or Automated Sanger method).

DNA sequencing is used to determine the nucleotides in a DNA strand. A sequencing method called dideoxy sequencing (also known as chain termination method or Sanger method) uses a primer as a start marker for the chain reaction.

Dideoxy sequencing (← links)
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See also: Sequencing, Sequence, DNA, Primer, Bases