Directional cloning. DNA insert and vector molecules are digested with two different restriction enzymes to create noncomplementary sticky ends at either end of each restriction fragment.
(See Directional cloning, Megabase cloning, Molecular cloning, Subcloning.) Coat protein (capsid). The coating of a protein that enclosed the nucleic acid core of a virus. Codon.
The vector's multiple cloning region (MCR) has several unique sites for both shotgun and directional cloning.
 See also: Cancer, Nucleotide, PCR, Virus, Polymerase
  
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