DNA Sequencing
DNA sequencing is the determination of the precise sequence of nucleotides in a sample of DNA.
DNA Sequencing and Sequence Variation
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DNA Sequencing
DNA sequencing is a laboratory technique used to determine the exact sequence of bases (A, C, G, and T) in a DNA molecule. The DNA base sequence carries the information a cell needs to assemble protein and RNA molecules.
DNA sequencing: Determining the order of bases in a segment of DNA.
DNA sequencing. Procedures for determining the nucleotide sequence of a DNA fragment.
DNase (deoxyribonuclease). See Nuclease.
[edit] DNA sequencing
Main article: DNA sequencing
DNA sequencing is the process of determining the nucleotide order of a given DNA fragment.
[edit] DNA sequencing and genomics
One of the most fundamental technologies developed to study genetics, DNA sequencing allows researchers to determine the sequence of nucleotides in DNA fragments.
DNA sequencing used to be done with these horrible polychrome gels and radioactivity. I did that for years when I was an undergrad and a graduate student.
the DNA sequencing of recombinant plasmids.
C.
the production of restriction endonuclease maps of recombinant plasmids.
Dideoxynucleotides are used in DNA sequencing and the treatment of viral diseases. (See Nucleotide.) didN. See Dideoxynucleotide. Digest. To cut DNA molecules with one or more restriction endonucleases. Diploid cell.
DNA sequencing -- "plus and minus" or "primed synthesis" method, developed by Sanger, ...
- A modifed form of dNTP used as a chain terminator in DNA sequencing
- A technique for identifying individual organisms based upon the uniqueness of their DNA pattern.
Gel-filled silica capillaries used to separate fragments for DNA sequencing.
Shortly after the methods of DNA sequencing were discovered, he sequenced 11 alleles of the enzyme alcohol dehydrogenase (AdH). Of the 43 polymorphic nucleotide sites he found, only one resulted in a change in the amino acid sequence of the protein.
Dr. Robert H. Lyons, Director
University of Michigan DNA Sequencing Core ...
Klenow fragment
A proteolytic fragment of DNA polymerase I that contains the DNA polymerase activity and the 3' to 5' proofreading activity but lacks the 5' to 3' exonuclease activity. This enzyme is widely used for DNA sequencing.
These nucleotides lack a 3'-OH (hydroxyl) group necessary for continued 5'-to-3' DNA synthesis. Dideoxynucleotides are used in molecular biology for Sanger-type DNA sequencing, ...
 See also: Sequencing, DNA, Genome, Human, Sequence
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