Dot blot
DNA/RNA or protein samples are spotted onto a membrane and hybridized with a labeled probe that hybridizes to a specific DNA/RNA or protein molecule. The technique is simple but has high background noise.
DOT BLOT - DNA or RNA is simply spotted onto nitrocellulose or nylon membranes, denatured and hybridized with a probe.
Dot blot: A technique for measuring the amount of one specific DNA or RNA in a complex mixture. The samples are spotted onto a hybridization membrane (such as nitrocellulose or activated nylon, etc.), fixed and hybridized with a radioactive probe.
After overcoating with THQ, biotinylated DNA was detected with a chemiluminescent dot blot assay. Polyethersulfone membranes exhibited the best performance in these assays.
Dot BlotThe simplest form of blot. Prepared by pipetting known volumes of DNA or RNA mixtures onto a blotting membrane.
The antibodies secreted by the different clones are then tested for their ability to bind to the antigen (for example with a test such as EIA or Antigen Microarray Assay) or immuno-dot blot, ...
 See also: DNA, Protein, Molecular biology, Biology, PCR
  
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