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Electrophoresis

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Electrophoresis is the most known electrokinetic phenomena. It was discovered by Reuss in 1809 [1]. He observed that clay particles dispersed in water migrate under influence of an applied electric field.

 


Electrophoresis
(Science: technique) separation of ionic molecules, (principally proteins) by the differential migration through a gel according to the size and ionic charge of the molecules in an electrical field.

electrophoresis
the differential movement of molecules through a gel under the influence of an electric field
Source: Jenkins, John B. 1990. Human Genetics, 2nd Edition. New York: Harper & Row ...

Electrophoresis
Electrophoresis is a laboratory technique used to separate DNA, RNA, or protein molecules based on their size and electrical charge. An electric current is used to move molecules to be separated through a gel.

Gel electrophoresis is a group of techniques used by scientists to separate molecules based on physical characteristics such as size, shape, or isoelectric point.

An electrophoresis technique for separation of large DNA. Alternating electrical field is applied to run DNA through a low density agarose gel matrix.
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Electrophoresis of DNA
DNA fragments have a constant charge/length ratio due to the net negative charge of the phosphate backbone. Therefore, DNA migrates toward the (+) electrode.

Electrophoresis, Supercomputing, and the Human Genome
The First International Conference on Electrophoresis, Supercomputing, and the Human Genome was held April 10-13 in Tallahassee, Florida.

Electrophoresis
The process in which molecules (such as proteins, DNA, or RNA fragments) can be separated according to size and electrical charge by applying an electric current to them.

Electrophoresis
Electrophoresis uses a direct electric current to separate the components of a mixture by the differing electrical charge.
Link to a description of how the proteins in blood serum are separated by electrophoresis.

Electrophoresis: A method of separating large molecules-such as DNA fragments or proteins-from a mixture of similar molecules.

Electrophoresis A technique whereby molecules are separated by movement in an electric field. In zone electrophoresis the molecules are ordered on the basis of their charge to weight ratios.

Electrophoresis. The technique of separating charged mol- ecules in a matrix to which is applied an electrical field. (See Agarose gell electrophoresis, Polycrylamide gell electrophoresis.) ...

Electrophoresis is a technique used to separate the DNA fragments according to their size. They are placed on a sheet of gelatin and an electric current is applied to the sheet.

Electrophoresis A technique used to separate charged molecules, such as proteins and nucleic acids, that is based on the fact that such molecules will move at differing rates in an electric field, depending on factors such as net charge, size, ...

gel electrophoresis
(jell eh-lek-troh-for-ee-sis)
The separation of nucleic acids or proteins, on the basis of their size and electrical charge, by measuring their rate of movement through an electrical field in a gel.
gene ...

Electrophoresis
- A technique of separating molecules based on their differential mobility in an electric field
Electroporation ...

electrophoresis The separation of different proteins or nucleic acids within a gel matrix that is subjected to an electric field; separation is based on size and/or charge of the molecule(s).

electrophoresis - separation technique in which an electric field is applied to a solution containing a protein molecule; the protein will migrate at a rate depending on its net
charge and on its size and shape.

Electrophoresis: See "Gel electrophoresis".

Endonuclease: An enzyme which digests nucleic acids starting in the middle of the strand (as opposed to an exonuclease, which must start at an end).

2D Gel Electrophoresis to Identify Cellular Proteins
While computer-based methods are powerful, they can only predict the function of proteins for which some information is already available.

AGAROSE GEL ELECTROPHORESIS - A method for separating nucleic acids (DNA or RNA) within a gel made of agarose in a suitable buffer under the influence of an electrical field.

Agarose gel electrophoresis
Following DNA digestion, the resulting DNA fragments are separated by size via electrophoresis in agarose gels. During electrophoresis, DNAs which are negatively charged migrate toward the positive electrode.

Polyacrylamide gel electrophoresis (PAGE)
Technique for separating protein components by molecular weight on the basis of passing through an acrylamide gel under the influence of an applied electric field.

Linus Pauling used electrophoresis to separate hemoglobin molecules. Sickle-cell anemia (h) is a recessive allele in which a defective hemoglobin is made, ultimately causing pain and death to those individuals homozygous recessive for the trait.

Agarose gel electrophoresis. A matrix composed of a highly purified form of agar that is used to separate larger DNA and RNA molecules ranging 20,000 nucleotides. (See Electrophoresis.) Alleles.

gel electrophoresis the process by which nucleic acids (DNA or RNA) or proteins are separated by size according to movement of the charged molecules in an electrical field.

Southern BlotDNA blot taken from an electrophoresis gel. The original blot type named after its originator Ed Southern, ...

Denaturing gradient gel electrophoresis (DGGE)
Denaturing high-performance liquid chromatography (DHPLC)
dendrites
Denhardt's solution
denominator element
density
density-gradient centrifugation
dentate nucleus ...

Short for Sodium Dodecyl Sulfate (SDS) - PolyAcrylamide Gel Electrophoresis (PAGE). Method to seperate proteins by exposing them to the anionic detergent SDS and PAGE.
Related Terms:
Electrophoresis ...

Denaturing gradient gel electrophoresis (DGGE)
A method for separating DNA fragments according to their mobilities under increasingly denaturing conditions (usually increasing formamide/urea concentrations).

A technique of blotting cloned DNA without prior restriction digestion and electrophoresis (See Southern blotting). Autoradiography reveals dots indicating probe hybridization.
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...

Agar is also a major microbiological media, and when purified, is a gel for electrophoresis. Agar is also used in food preparation to keep baked goods from drying and to set jellies, and desserts.

Using the then new technique of protein electrophoresis, they showed that 30% of the loci in a population of Drosophila pseudoobscura were polymorphic. They also showed that it was likely that they could not detect all the variation that was present.

amplification using primers complementary to the adaptor sequences. The multiple rounds of amplification are used to reduce the complexity of the PCR product population so that the amplified fragments can be easily resolved by gel electrophoresis.

See also: DNA, Gel, Sequence, Trans, Protein