Electroporation
A method for transferring DNA (or other small molecules) into cells by exposure to a rapid pulse of high voltage, which causes the transient formation of small pores in the cell membrane.
Electroporation involves using an electric current to create pores in the cell wall and plasma membrane for DNA to enter.
Electroporation. A method for transforrning DNA, especially useful for plant cells, in which high voltage pulses of electricity are used to open pores in cell membranes, through which foreign DNA can pass.
Electroporation A process using high-voltage current to make cell membranes permeable to allow the introduction of new DNA; commonly used in recombinant DNA technology.
See also: transfection ...
Electroporation
- Technique for introducing DNA into cells by giving a transient electric pulse
Endonuclease ...
Electroporation is another way to make holes in bacterial (and other) cells, by briefly shocking them with an electric field of 10-20kV/cm. Plasmid DNA can enter the cell through these holes. This method is amenable to use with large plasmid DNA.
(See Agarose gell electrophoresis, Polycrylamide gell electrophoresis.) Electroporation.
Methods for delivering DNA/RNA into tissue culture cells, include lipofection, electroporation, and calcium phosphate transfection.
Related
Stable transfection Transient transfection ...
The cells are then briefly heat shocked so the plasmid can cross the plasma membrane. An alternate method, electroporation, uses a short electrical pulse to open pores in the plasma membrane, allowing the plasmid to pass through.
 See also: Trans, DNA, Cells, Sequence, Protein
  
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