Endonuclease

endonuclease
An enzyme that catalyzes a break at some point within the DNA molecule.
Source: Jenkins, John B. 1990. Human Genetics, 2nd Edition. New York: Harper & Row ...

Endonuclease
Endonuclease enzyme digests nucleic acids starting in the middle of the strand.
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endonuclease that initiates excision repair at apurinic and apyrimidinic sites on DNA.
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...

AP endonuclease
Apurinic/apyrimidinic (AP) endonuclease is an enzyme that is involved in the DNA base excision repair pathway (BER). Its main role in the repair of damaged or mismatched nucleotides in DNA is to create a nick in the...

Endonuclease
An enzyme that makes breaks in a molecule of DNA by hydrolyzing internal phosphodiester bonds. An endonuclease may be specific for either single or double stranded DNA or RNA.

Endonuclease. See Nuclease.
Endophyte. An organism that lives inside another.

Endonuclease: A nuclease which cuts a nucleic acid molecule by cleaving the phosphodiester bonds between two internal residues. Best known examples are restriction endonucleases.

Endonuclease See: restriction enzyme
Enzyme A protein that acts as a catalyst, speeding the rate at which a biochemical reaction proceeds but not altering the direction or nature of the reaction.

Endonuclease
- An enzyme that hydrolyzes internal phosphodiester bonds in a polynucleotide
Enhancer ...

ENDONUCLEASE - Cleaves bonds within a nucleic acid chain; they may b especific for RNA or for single-stranded or double-stranded DNA. A restriction enzyme is a type of endonuclease.

Endonuclease: An enzyme which digests nucleic acids starting in the middle of the strand (as opposed to an exonuclease, which must start at an end). Examples include the restriction enzymes, DNase I and RNase A.
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an endonuclease that cuts DNA and a
reverse transcriptase that makes a DNA copy of an RNA transcript.
L1 activity proceeds as follows: ...

Restriction endonuclease generated DNA fragments separated by gel electrophoresis and blot transferred onto a membrane filter are probed with a radioactive DNA fragment. This procedure is called:
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Gene cloning ...

restriction endonuclease. An enzyme that cleaves a DNA molecule at a particular base sequence.

restriction endonuclease - aka restriction enzyme - bacterial enzyme that cuts double-stranded DNA at a specific sequence.

Restriction endonucleases, (restriction enzymes) Bacterial nucleases which cut double stranded DNA at specific sites defined by short (4- 10bp) usually palindromic sequences.

Synonym: nucleate endonuclease, nuclease o, endonuclease (serratia marcescens), nuclease sm, nuclease sm1, nuclease sm2, extracellular nuclease, serratia marcescens ...

To cut DNA molecules with one or more restriction endonucleases. Diploid cell. A cell which contains two copies of each chromosome. See Haploid cell. Directional cloning.

The name is derived from the fact that these sequences are cleaved by the restriction endonuclease Alu. Amino acid sequence -- the linear order of the amino acids in a protein or peptide.

The DNA is then cut into restriction fragments using suitable endonucleases, which only cut the DNA molecule where there are specific DNA sequences, termed recognition sequence or restriction sites, that are recognized by the enzymes.

Restriction enzymeMore correctly called a Type II Restriction Endonuclease. A bacterial enzyme which forms part of a system to protect the cell against infection by bacteriophage, and unregulated influx of foreign DNA.

Restriction enzyme, endonuclease A protein that recognises specific, short nucleotide sequences and cuts DNA at those sites. Bacteria contain over 400 such enzymes that recognise and cut over 100 different DNA sequences.

Landmarks include restriction endonuclease recognition sites, DNA sequence and chromosomal bands. Distance is measured in base pairs.

At the entry site, endonucleases cut the DNA into fragments of 7,000-10,000 nucleotides, and the double-stranded DNA separates into single strands. The single-stranded DNA may recombine with the host's chromosome once inside the cell.

Diabetes mellitus is the inability of beta cells of pancreas to produce insulin
Restriction enzymes/endonuclease cut DNA at specific recognition sites
This produces either "sticky ends" or "blunt ends"
DNA ligase can be used to re-join the ends ...

Attachment of methyl groups (-CH3) to DNA most commonly at cytosine residues. May be involved in regulation of gene expression. Also may prevent some restriction endonucleases from cutting DNA at their recognition sites.
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Restriction enzymes Endonuclease enzymes that recognize specific base sequences in double-stranded DNA and cleave both strands of the duplex at specific places.

See also: DNA, Nuclease, Sequence, Enzyme, Restriction