Ethidium Bromide

Ethidium bromide
A fluorescent chemical that intercalates between base pairs in a doublestranded DNA molecule. Commonly used to detect DNA following gel electrophoresis. Ethidium bromide intercalates between the stacked bases of DNA and RNA.

Ethidium bromide. A fluorescent dye used to stain DNA and RNA. The dye fluoresces when exposed to UV light.

Ethidium bromide
- A molecule that binds to DNA and fluoresces when viewed under ultraviolet light. Used as a stain for DNA.
Ethylene ...

Figure 3: Ethidium bromide-stained PCR products after gel electrophoresis. Two sets of primers were used to amplify a target sequence from three different tissue samples.

Ethidium bromide, Silver, or Coomassie blue dye can be used. Other methods can also be used to visualize the separation of the mixture's components on the gel.

The DNA bands must be stained to make them visible. Ethidium bromide-stained DNA will fluoresce when illuminated with UV light.
PCR techniques are used to produce sufficient quantities of DNA for this technique.
Southern Blotting ...

Gels are stained with ethidium bromide and images are captured on the Biorad Fluor-S MultiImager system and scored using the Bio Image Advanced Quantifier software.

Intercalating agents Flat, aromatic compounds that can insert between adjacent base pairs in a DNA double helix; these agents, such as ethidium bromide, can cause insertions and deletions.

fragments at four different discrete sizes, those four size classes will, when subjected to electrophoresis, all migrate in groups, producing four migrating "bands". Usually, these are visualized by soaking the gel in a dye (ethidium bromide) which ...

See also: DNA, Strand, Sequence, Trans, Bases