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Expressed sequence tag

Biology  Expressed gene  Expression

Expressed sequence tag
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An expressed sequence tag or EST is a short sub-sequence of a transcribed protein-coding or non-protein coding DNA sequence. It was originally intended as a way to identify gene transcripts, but has since been instrumental in gene discovery and sequence determination.

Expressed Sequence Tags (dEST): dbEST (Nature Genetics 4:332-3;1993) is a division of GenBank that contains sequence data and other information on "single-pass" cDNA sequences, or Expressed Sequence Tags, from a number of organisms.

Expressed sequence tag (EST) is STS derived from cDNA.
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Expressed Sequence Tags (ESTs)
ESTs are short (200-500 nucleotides) DNA sequences that can be used to identify a gene that is being expressed in a cell at a particular time.
The Procedure: ...

~ (EST). Partial or complete cDNA sequence.
Expressivity. The observed quantitative differences in the expression of a phenotype among individuals that have the same mutant genotype. When quantitative differences are observed, the phenotype has variable expressivity.

~ (EST) A short portion of a cDNA molecule serving to identify a gene. Used in locating genes on chromosomes. See also: sequence tagged site.
extremophile /ig-STREM-ə-file/ An organism that thrives under extreme conditions, such as extreme heat or salinity.

~ (EST)
A short strand of DNA that is a part of a cDNA molecule and can act as identifier of a gene. Used in locating and mapping genes.
See also: cDNA, sequence tagged site
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EST (~): A partial sequence of a cDNA molecule. See also STS.
Ethology: Study of animal behaviour under normal conditions.

~ analyses of the annelid Pomatoceros lamarckii, recently published in BMC Evolutionary Biology, are consistent with less extensive gene loss in the Lophotrochozoa than in the Ecdysozoa, ...

EST: ~
NCBI: National Center for Biotechnology Information
RNA-seq: RNA sequencing ...

ESTs, "~s"
STRPs, "short tandem repeat polymorphisms" ...

EST: ~
Flanking Region: For microsatellites, the flanking regions are the stretches of DNA outside the simple sequence tandem repeat (STR). These sequences are used as primer pairs.

See: gene expression (ORNL)
~ (EST)
A short strand of DNA that is a part of a cDNA molecule and can act as identifier of a gene. Used in locating and mapping genes.
See also: cDNA, sequence tagged site (ORNL) ...

~s (ESTs) are STSs derived from cDNAs.
Sequencing Determination of the order of nucleotides (base sequences) in a DNA or RNA molecule or the order of amino acids in a protein.

1991 Sequencing of human ~s begins in Craig Venter's lab, an attempt to capture the coding fraction of the human genome.

clone seq Clones that are mapped onto the genome sequence include annotation units (e.g. BACS used to assemble the genome sequence), full length cDNAs and ~s. The clone sequence is matched to the genome sequence according to a set of parameters defined for each clone type.

Any unique DNA sequence which can be used in DNA hybridization, PCR or restriction mapping experiments to identify that sequence. See ~ and sequence tagged site.

Special software scans the sequences for the telltale signs of protein-coding genes, looking for start and stop signals, RNA-splicing sites, and other features.
The software also looks for ~s (ESTs), sequences similar to those in known genes.

See also: See also: What is the meaning of Express, Sequence, Genome, Human, DNA?

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