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F Factor

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F factor
A fertility factor in bacteria, a DNA segment that confers the ability to form pili for conjugation and associated functions required for the transfer of DNA from donor to recipient.

 


F Factor (Fertility Factor): Transmissible plasmid (episome) in bacteria (such as E. coli) that acts as a sex factor. It is a circular DNA about 94 kb long. Conjugation and chromosomal gene transfer occur from F+ (male) to F- (female) bacterium.

This occurs because the F factor has integrated itself via an insertion point in the bacterial chromosome.

Problem 5: DNA transfer by an F factor
An E. coli strain is F lac Z met bio. Cells from this strain are mixed with an E.

These clones are propagated at very low copy number under control of the replication and partitioning functions of the F factor, which helps to stabilize potentially toxic clones.

it is more likely that host genes close behind the leading F genes ("locomotive") will make it than those farther back
The "caboose" seldom makes it so failing to receive a complete F factor, the recipient cell continues to be "female" ...

See also: Trans, DNA, Cells, Bacteria, Cell

Biology EyespotsF1

 
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