Flanking region. The DNA sequences extending on either side of a specific locus or gene.
3' flanking region: A region of DNA which is NOT copied into the mature mRNA, but which is present adjacent to 3' end of the gene (see Figure 4).
Microsatellites can be amplified for identification using Polymerase Chain Reaction (PCR), using templates of flanking regions (primers).
Primers constructed from the flanking regions of microsatellite DNA which can be used in PCR reactions to amplify the repeat region.
Flanking sequence or flanking region
The DNA sequence located on either side of a specific genetic locus.
Polymerase chain reaction A method for amplifying DNA sequences by using DNA polymerase; a series of three-step cycles is employed, in which parental DNA strands are separated by heating, primers to flanking regions of the target sequence are ...
The 5' flanking regions of the gene in both species shows a similar high degree of conservation. In general, the analysis of a 400 bp segment preceding the initiator ATG shows only a 60% similarity.
 See also: DNA, Molecule, Trans, Gene, Sequence
  
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