Footprinting
A method for identification of a protein-binding site on a DNA molecule.
Footprinting, DNAase: DNA with protein bound is resistant to digestion by DNAase. When a sequencing reaction is performed using such DNA, a protected area representing the footprint of the bound protein will be detected.
Footprinting A technique used to determine the site of a protein that binds to DNA. DNA is digested in the presence or absence of the protein, and the sequence protected from digestion by the protein is identified.
FOOTPRINTING - A technique for identifying the site on a DNA (or RNA) molecule which is bound by some protein by virtue of the protection afforded phosphodiester bonds in this region against attack by nuclease or nucleolytic compounds.
Footprinting: A technique by which one identifies a protein binding site on cellular DNA. The presence of a bound protein prevents DNase from "nicking" that region, which can be detected by an appropriately designed gel.
...
"From genetic footprinting to antimicrobial drug targets: examples in cofactor biosynthetic pathways". J. Bacteriol. 184 (16): 4555-72. PMID 12142426.
^ Senkovich O, Speed H, Grigorian A, et al (2005).
Techniques such as chemical protection and enzymatic footprinting together with gel-retardation assays enable the mapping of proteins on genomic DNA but provide no information on bound proteins.
Learn the sequence to which the repressor binds (by footprinting).
Link to a discussion of footprinting.
In Allele- specific PCR the opposite is done. ... In vivo footprinting of a muscle specific enhancer by ligation mediated PCR" ...
Full article ...
 See also: DNA, Trans, Sequence, Protein, Organ
  
|
RSS
