G-banding is obtained with Giemsa stain following digestion of chromosomes with trypsin. It yields a series of lightly and darkly stained bands - the dark regions tend to be heterochromatic, late-replicating and AT rich.
Giemsa banding (G-banding)
Method of staining chromosomes that produces light and dark bands characteristic for each chromosome. Bands are produced by staining with Giemsa stain after pretreating chromosomes with trypsin.
Composed of repetitive DNA, stains dark bands in G-banding. (Cf. Euchromatin.)
Related Terms:
Chromosome
The term was proposed by Waldeyer (1888) for the individual threads within a cell nucleus (gk. chroma, colour; soma, body).
We prepared a hybrid cell line containing human chromosomes having a deletion that maps to 5q31-5q33 by G-banding.
In the "classic" (depicted) karyotype, a dye, often Giemsa, is used to make bands on the chromosomes visible. This is also referred to as G-banding.
One common dye used is Giemsa; That process is known as G-banding (see the G-banded chromosomes in the image to the left).
 See also: Chromosomes, Chromosome, Cell, Cells, DNA
  
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