Gene splicing: The isolation of a gene from one organism and then the introduction of that gene into another organism using techniques of biotechnology.
Gene splicing. Combining genes from different organisms into one organism. See recombinant DNA.
Gene translocation. The movement of a gene fragment from one chromosomal location to another, which often alters or abolishes expression.
Gene splicing and use of recombinant DNA (rDNA) are major techniques used. Biotic stress. Living organisms which can harm plants , such as viruses, fungi, and bacteria, and harmful insects. See Abiotic stress. bP. See Base pair. Bt.
With gene splicing, the promoter of a gene whose expression you wish to monitor can be coupled to the coding sequence of a chosen "reporter" gene.
lacZ
One favorite reporter gene is the Z gene of the E.
Phillip Sharp (Massachusetts Institute of Technology) and Richard Roberts (formerly Cold Spring Harbor Laboratory, now New England Biolabs) were jointly awarded the 1993 Nobel Prize in Medicine and Physiology for their 1977 work on gene splicing.
In the same year, Richard Roberts and Phillip Sharp independently showed that the genes of adenovirus contain introns and therefore require gene splicing. It was later realized that almost all genes of eukaryotes have introns as well.
 See also: Gene, Strand, Protein, Trans, Cells
  
|
RSS
