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Genomic DNA

Biology Genomic cloneGenomic imprinting

Genomic DNA DNA derived from the genome, as distinct to cDNA. It may have been directly purified but it may also have been manipulated in some way, by PCR for instance or by cloning.

 


genomic DNA - nuclear DNA of the chromosomes.
genomic equivalence - concept that each cell in the body has the same genetic material and therefore all the information necessary to create a complete organism.

Genomic DNA is located in the cell nucleus of eukaryotes, as well as small amounts in mitochondria and chloroplasts. In prokaryotes, the DNA is held within an irregularly shaped body in the cytoplasm called the nucleoid.

Directed Genomic DNA Sequencing on Human Chromosome 5
Christopher H. Martin, Cheryl A. Davis, Cheryl L. Ericsson, Carol A. Mayeda, Herb Moise, and Michael J. Palazzolo ...

genomic_DNA Genomic DNA includes all nuclear, mitochondrial and plastid DNA. Genomic DNA stocks from the ABRC are derived from an individual line.

2.1 Isolation of genomic DNA
2.2 Amplification and quantitation of DNA
3 Variations on the basic PCR technique
4 History ...

Satellite DNA A genomic DNA fraction which has a different density to the main body of genomic DNA and therefore forms a separate band (satellite) during density gradient ultracentrifugation.

A library composed of fragments of genomic DNA. (See Library.) Genotype. The structure of DNA that determines the expression of a trait. See Phenotype. Genus. A category including closely related species.

RFLPs have been very useful to use as markers for following a genomic DNA, either from human or other animals. What is it, though?

Techniques developed for analyzing genomic DNA (fingerprinting) of higher organisms. It is based on the principle that restriction enzymes cutting sites can be uses as landmarks.

Genomic blot: A type of Southern blot specifically used to analyze a mixture of DNA fragments derived from total genomic DNA.

Short (200 to 500 base pairs) sequence of genomic DNA that has a single occurrence in the human genome and whose location and base sequence are known.

genomic DNA) is separated by electrophoresis in agarose gel and transferred onto a membrane. The membrane is then probed with labeled DNA to detect the specific sequence. Named after its inventor, Dr. Southern.

Genomics
The comparative analysis of genomic DNA sequences from different organisms. Genomic analysis can provide information about the evolution of genes and can make predictions about the metabolism of an organism.

Genomic library. A library composed of fragments of genomic DNA. (See Library.)
Genotype. The structure of DNA that determines the expression of a trait. See Phenotype.

plasmid A small circle of DNA that may be carried by a bacterium in addition to its genomic DNA.
plasmodesma A cytoplasmic strand that connects adjacent plant cells through pores in the cell wall.
plasmodium Multinucleate ameboid mass, syncytial.

In eukaryotes, genomic DNA is located in the cell nucleus, along with small amounts in mitochondria and chloroplasts. In prokaryotes, the DNA is held within an irregularly shaped body in the cytoplasm called the nucleoid.

- Term that denotes the differences in molecular weight of homologous fragments of restriction enzyme-digested genomic DNA sometimes observed in two genetically distinct individuals
Ribosomal RNA/rRNA ...

complementary DNA (CDNA) - DNA molecule made as a copy of mRNA and therefore lacking the introns that are present in genomic DNA
cristae - folds of the inner mitochondrial membrane ...

Southern blotting allows the detection of fragments of genomic DNA, which anneal to the radiolabeled probe. The fragments are generated using restriction enzymes and separated in a gel by electrophoresis.

Complementary (copy) DNA (cDNA): Single-stranded DNA produced from an RNA template (usually mRNA) by reverse transcriptase in vitro. It lacks the introns present in corresponding genomic DNA.

See also: DNA, Sequence, Genome, Human, Trans