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The his tag can also be used in detection of the protein via anti-his-tag antibodies. This can be useful in localization, ELISA, western blots or other immunoanalytical methods.
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A simple column for Ni2+-affinity chromatography. The sample and subsequent buffers are manually poured into the column.
 See also: Antibodies, Fusion Protein, Chromatography, Residue, Affinity chromatography
  
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