hybridoma
a hybrid cell made from the fusion of a cancer cell with a lymphocyte that has been induced to produce antibodies
Source: Jenkins, John B. 1990. Human Genetics, 2nd Edition. New York: Harper & Row ...
Hybrid
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Hybridisation
In genetics, hybridisation is the process of mixing different species or varieties of organisms.
In molecular biology hybridisation is the process of joining two complementary strands of DNA.
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Definition of trihybrid :
An organism heterozygous at three loci.
Hybridization
Hybridization is the process of combining two complementary single-stranded DNA or RNA molecules and allowing them to form a single double-stranded molecule through base pairing.
Monohybrid Cross Problem Set
Genetics is the study of heredity and variation in organisms. We begin with a study of the monohybrid cross, invented by Mendel. In a monohybrid cross, organisms differing in only one trait are crossed.
In situ hybridization
(Science: molecular biology) The use of a dna or rna probe to detect the complementary dna sequence.
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A hybrid cell containing small fragments of irradiated human chromosomes.
Hybridization Data
Uwe Maskos (NIH) opened a session chaired by Southern with a forthright discussion of the challenges of interpreting real data obtained by hybridization of target DNA with oligonucleotide arrays.
Hybridization
A technique where a denatured (single-stranded) nucleotide chain (DNA or RNA) is allowed to pair with another single-stranded nucleotide chain.
hybrid
[L. hybrida, the offspring of a tame sow and a wild boar]
(1) Offspring of two parents that differ in one or more inheritable characteristics. (2) Offspring of two different varieties or of two different species.
hybrid zone ...
Hybrid: Seed or plants produced as the result of controlled cross-pollination as opposed to seed produced as the result of natural pollination. Hybrid seeds are selected to have higher quality traits (for example, yield or pest tolerance).
Hybridization: The process of joining two complementary strands of DNA or one each of DNA and RNA to form a double-stranded molecule.
I ...
Hybridisation Used in molecular biology to describe the process of annealing one strand of a polynucleotide (DNA or RNA), the probe, to a strand of complementary sequence (again either DNA or RNA), the target.
Hybridization. The hydrogen bonding of complementary DNA and/or RNA sequences to form a duplex molecule. (See Northern hybridization, Southern hybridization.) ...
Hybridization A technique used to determine the relatedness of nucleic acids by assaying the ability of single strands of one sample to form a duplex by complementary base pairing to single stands of another sample.
dihybrid cross In genetics, a cross that involves two sets of characteristics. PICTURE ...
monohybrid cross In genetics, a cross that involves only one characteristic. PICTURE ...
So the hybrid was the human cell and the mouse cell?
Right. Because things were fluid in the membrane [of the hybrid cell], basically the mouse and human content gets uniformly disseminated on the surface of that hybrid cell.
DNA-DNA Hybridization
As we saw in the comparison of human and kangaroo cytochrome c, a single molecule provides only a narrow window for glimpsing evolutionary relationships.
in situ hybridization - detection of the location of nucleic acid sequences in a cell or organism.
inducer gene - gene encoding the repressor protein of the lac operon; when lactose binds the repressor protein, the lac operon is induced.
Hybridoma
- A type of hybrid cell produced by fusing a normal cell with a tumor cell.
Hybrid
A cross-bred, heterozygot organism or cell, an individual from any cross involving parents of differing genotypes. Offspring of unlike parents..
In molecular genetics a DNA molecule with strands of different origin. Cf. hybridisation.
Hybridization conditions: higher the stringency, lower the probability of hybridization. Increase the temperature or decreasing the salt concentration raises the stringency.
Related
Hybridization Melting temperature ...
HYBRIDOMA - A clone of plasmacytoma cells which secrete a monoclonal antibody; usually produced by fusion of peripheral or splenic plasma cells taken from an immunized mouse with an immortalized murine plasmacytoma cell line (fusion partner), ...
hybrid Offspring of a cross between two species or between alternate homozygous conditions.
hybridoma Fused product of a normal and a myeloma (cancer) cell, which has some of the characteristics of the normal cell.
Hybridization: The reaction by which the pairing of complementary strands of nucleic acid occurs. DNA is usually double-stranded, and when the strands are separated they will re-hybridize under the appropriate conditions.
DNA hybridization (exploiting the fundamental principle of complementary base pairing) studies have been used to reveal the relationships between species that could not be resolved by other means.
Orbital Hybridization
When an atom forms covalent bonds, the s orbital and the p orbitals of the valence shell may become rearranged to form four new hybrid orbitals. The red structures in the model below represent the hybridized orbitals.
Replicate Hybridization The set of hybridizations that are performed with similar samples and arrays, which can be averaged. Replicates can be technical or biological.
DNA hybridization -- a technique for selectively binding specific segments of single-stranded (ss) DNA or RNA by base pairing to complementary sequences on ssDNA molecules that are trapped on a nitrocellulose filter.
In the authors' hybrid GA, a shortest-path-first algorithm, which minimizes the number of "hops" a given data packet must pass through, is used to generate the seed for the initial population.
DNA renaturation (hybridization) - process whereby two complementary nucleic acid strands form a double helix during an annealing period; a powerful technique for detecting specific nucleotide sequences ...
Cross-hybridization. The hydrogen bonding of a single- stranded DNA sequence that is partially but not entirely complementary to a singlestranded substrate.
This is necessary prior to probe hybridisation and most methods involving enzymic DNA synthesis on a DNA template e.g. sequencing, PCR, some labelling methods. Denaturation may be achieved by heating or treatment with NaOH.
SBH sequencing by hybridization
SBIR Small Business Innovation Research
SCAN Sequence Comparison ANalysis Program
SCE School of Continuing Education
SCI Society of Chemical Industry
SCW single-chromosome workshop ...
In most NFT-bearing neurons, we observed a strong reduction in acetylated a-tubulin immunoreactivity (a marker of stable microtubules) and a reduction of the in situ hybridization signal for tubulin mRNA.
(fluorescence in situ hybridization): One of the more modern methods in cytogenetics, which uses fluorescence-labelled chromosome-specific DNA, probes to detect translocations, inversions, deletions, ...
FISH (fluorescence in situ hybridization): A mapping technique that uses fluorescent tags to identify specific locations of chromosomes.
reticulation -- Joining of separate lineages on a phylogenetic tree, generally through hybridization or through lateral gene transfer. Fairly common in certain land plant clades; reticulation is thought to be rare among metazoans.
Recombinant DNA is produced when genetic information from more than one organism is recombined in a laboratory process into a hybrid molecule.
Source : PhRMA Genomics
Replication
The process by which DNA is duplicated before cell division.
Crossing over A process that occurs during meiosis in which chromosome partners, one inherited from each parent, physically swap sections with one another. This creates hybrid chromosomes that are a patchwork of the original pair.
Label two chromatids with each letter. This way they can line up the pairs of homologous chromosomes during metaphase I and demonstrate all of the phases of meiosis. This leads right into a lesson on dihybrid crosses and probability.
However, the virus replaces the proteins in the cell membrane with its own proteins, creating a hybrid structure of cell-derived lipids and virus-derived proteins.
 See also: DNA, Human, Trans, Organ, Sequence
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