Association of two complementary nucleic acid strands to form double-stranded molecules, which can contain two DNA strands, two RNA strands, or one DNA and one RNA strand. Used experimentally in various ways to detect specific DNA or RNA sequences.
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Hybridization is the process of combining two complementary single-stranded DNA or RNA molecules and allowing them to form a single double-stranded molecule through base pairing.
In molecular biology, refers to the process by which sample DNA attached to a matrix (usually a membrane) is incubated with a labelled probe. The probe will anneal to the sample DNA where there is sufficient complementarity.
in situ hybridization
locating a gene by adding specific radioactive probes for the gene and detecting the location of the radioactivity on the chromosome after hybridization
Source: Jenkins, John B. 1990. Human Genetics, 2nd Edition. New York: Harper & Row ...
In situ hybridization
(Science: molecular biology) The use of a dna or rna probe to detect the complementary dna sequence.
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single-stranded nucleic acid sequences by hydrogen bonding of complementary bases to form double-stranded molecules; this process is the basis for molecular biological techniques in which a labeled probe sequence is used to detect another identical or similar sequence (e.g., Southern hybridization, ...
A technique where a denatured (single-stranded) nucleotide chain (DNA or RNA) is allowed to pair with another single-stranded nucleotide chain.
~ is effective pollination between flowers of different species of the same genus, or even between flowers of different genera (as in the case of several orchids).
~ /HIGH-brid-eye-ZAY-shən, -brəd-/ n. (1) interbreeding between distinct genetic types, including types treated as distinct species; (2) the process of joining two complementary strands of DNA, or one each of DNA and RNA, to form a double-stranded molecule.
~: The specific reassociation of complementary strands of nucleic acids.
Hybrid vigor (heterosis): Unusual growth, strength, and health of heterozygote offspring from two less vigorous homozygote parents.
The process of joining two complementary strands of DNA or one each of DNA and RNA to form a double-stranded molecule.
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~ with radioactive probe
A single locus probe is a DNA or RNA sequence that is able to hybridize (i.e. form a DNA-DNA or DNA-RNA duplex) with DNA from a specific restriction fragment on the Southern blot.
DNA hybridization The formation of hybrid DNA molecules that contain a strand of DNA from two different species. The number of complementary sequences in common in the two strands is an indication of the degree of relatedness of the species.
As we saw in the comparison of human and kangaroo cytochrome c, a single molecule provides only a narrow window for glimpsing evolutionary relationships.
In situ hybridization A technique for mapping cloned DNA sequences by hybridization directly to metaphase chromosomes and analysis by microscopy (see Chapter 10).
See In situ Hybridization in the MGI Glossary.
In situ hybridization and immunohistochemistry
In situ hybridization and immunohistochemistry were sequentially performed on the same section. Riboprobes were derived from DNA fragments obtained by PCR from E12.5 MGE cDNA using the primers listed in Additional file 4.
in situ hybridization - detection of the location of nucleic acid sequences in a cell or organism.
inducer gene - gene encoding the repressor protein of the lac operon; when lactose binds the repressor protein, the lac operon is induced.
In situ hybridization
For analysis of endogenous gene expression, wild-type and transgenic lungs at E18.5 were fixed in 4% paraformaldehyde/phosphate-buffered saline, dehydrated and embedded in paraffin, and 7 μm sections cut.
~ conditions: higher the stringency, lower the probability of hybridization. Increase the temperature or decreasing the salt concentration raises the stringency.
~ Melting temperature ...
- Production of offspring, or hybrids, from genetically dissimilar parents. In selective breeding, it usually refers to the offspring of two different species.
~ in Genetics is the crossing of individuals from 'pure' and different lineages in relation to a given trait, i.e., the crossing of different homozygous for the studied trait.
~: The reaction by which the pairing of complementary strands of nucleic acid occurs. DNA is usually double-stranded, and when the strands are separated they will re-hybridize under the appropriate conditions. Hybrids can form between DNA-DNA, DNA-RNA or RNA-RNA.
A ~ technique that enables researchers to determine the presence of certain nucleotide sequences in a sample of DNA.
(1) Of cells, having particular functions in a multicellular organism. (2) Of organisms, having special adaptations to a particular habitat or mode of life.
DNA hybridization (exploiting the fundamental principle of complementary base pairing) studies have been used to reveal the relationships between species that could not be resolved by other means.
In situ hybridization of a genome. This technique allows the discrimination between chromosomes originating from different species present within one nucleus. GISH can also be used to study the meiosis of the somatic hybrids and the back-cross hybrids.
In situ hybridization ...
In situ hybridization
Use of a DNA or RNA probe to detect the presence of the complementary DNA sequence in chromosome spreads or in interphase nuclei or an RNA sequence of cloned bacterial or cultured eukaryotic cells.
In-situ hybridization is a process that allows us to see which cells are expressing a particular mRNA. The way we do that is we make a probe that is similar to that mRNA, but it's an anti-sense probe. That is, the sequence of nucleotides are the ones that bind to the original mRNA.
...aste, and replace with ... Molecular Research Center develops Hybridization solutions , including High Efficiency hybridization systems, Super hyb Kit, formazol, for Southern, northern, dot ... ...
Replicate Hybridization The set of hybridizations that are performed with similar samples and arrays, which can be averaged. Replicates can be technical or biological.
DNA hybridization -- a technique for selectively binding specific segments of single-stranded (ss) DNA or RNA by base pairing to complementary sequences on ssDNA molecules that are trapped on a nitrocellulose filter.
~. is used to determine the presence of a specific nucleotide sequence. Labeled probes complementary to the gene of intrest are allowed to bind to DNA from the cells being tested. This will determine if the gene being sought is present.
This spontaneous ~ may lead to a 'superweed,' which may have a selective advantage and be difficult to control.
Some crops do hybridize with weedy relatives, and crop-to-weed transgene escape is a possibility.
At the request of ~ experiment,a design of intelligent ~ oven base on expert PID control and embedded operation system μC/OS-II.
DNA renaturation (~) - process whereby two complementary nucleic acid strands form a double helix during an annealing period; a powerful technique for detecting specific nucleotide sequences ...
Speciation through ~ and/or polyploidy has long been considered much less important in animals than in plants [[[refs.]]]. A number of reviews suggest that this view may be mistaken. (Lokki and Saura 1980; Bullini and Nascetti 1990; Vrijenhoek 1994).
Cross-~. The hydrogen bonding of a single- stranded DNA sequence that is partially but not entirely complementary to a singlestranded substrate. Often, this involves hybridizing a DNA probe for a specific DNA sequence to the homologous sequences of different species. Cross-pollination.
Smaller variants, particularly CNVs, segmental duplications, and interchromosomal interstitial rearrangements, are assessed by fluorescence in situ ~ (FISH) or competitive genomic ~ arrays (aCGH).
Syntenic — Describing genetic loci that reside on the same chromosome.
1859 Charles Darwin publishes The Origin of Species 1865 Gregor Mendel's paper, Experiments on Plant ~ 1903 Chromosomes are discovered to be hereditary units 1905 British biologist William Bateson coins the term "genetics" in a letter to Adam Sedgwick 1910 Chromosomes include genes 1913 ...
FISH (fluorescence in situ ~) A physical mapping approach that uses fluorescein tags to detect ~ of probes with metaphase chromosomes and with the less-condensed somatic interphase chromatin.
(fluorescence in situ ~): One of the more modern methods in cytogenetics, which uses fluorescence-labelled chromosome-specific DNA, probes to detect translocations, inversions, deletions, amplifications and other structural or numerical chromosomal abnormalities.
Plants can also do this kind of gene transfer if sometimes if you have ~ and this can happen in another kind of creatures besides simply plants but ~ when you have two species cross sometimes you windup adding some genes from a new spec- from the old species unto the new ...
by DNA sequence comparison or ~) to a particular spot in the yeast genome. A locus may also be a DNA sequence feature such as a centromere.
FISH (fluorescence in situ ~): A mapping technique that uses fluorescent tags to identify specific locations of chromosomes.
Gel: A dense net work of fine particles dispersed with water (Jell-O is a gel). Used to separate different-sized strands of DNA.
Minisatellite technology relies on probe-based ~. Advantages include lack of need for specific primers and hypervariability.
The formation of novel genetic combinations through ~ of genetically distinct groups.
See amplified fragment length polymorphism.
gene flow An exchange of genes between two populations of a species, or in extreme cases, between populations of two species (~).
gene insertion The process by which one or more genes from one organism are incorporated into the genetic makeup of a second organism.
Chromosome painting. Use of differentially labeled, chromosome-specific DNA strands for ~ with chromosomes to label each chromosome with a different color.
NHGRI Narrower terms: autosome, centromere, chromatin, euchromatin, heterochromatin, homologous chromosomes, telomere Related terms chromosome maps, cytogenetics, diploid, euchromatic, haploid, karyotype, ploidies, ploidy, somatic cell ~ ...
See also: What is the meaning of Hybrid, DNA, Trans, Sequence, Biology?