In situ hybridization

in situ hybridization
locating a gene by adding specific radioactive probes for the gene and detecting the location of the radioactivity on the chromosome after hybridization ...

In situ hybridization
(Science: molecular biology) The use of a dna or rna probe to detect the complementary dna sequence.
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In Situ Hybridization
In situ hybridization is a laboratory technique in which a single-stranded DNA or RNA sequence called a probe is allowed to form complementary base pairs with DNA or RNA present in a tissue or chromosome sample.

In situ hybridization
A technique for gene mapping involving hybridization of a labelled sample of a cloned gene to a large DNA molecule (usually a chromosome), often within a cell.

in situ hybridization - detection of the location of nucleic acid sequences in a cell or organism.
inducer gene - gene encoding the repressor protein of the lac operon; when lactose binds the repressor protein, the lac operon is induced.

In situ hybridization
Use of a DNA or RNA probe to detect the presence of the complementary DNA sequence in cloned bacterial or cultured eukaryotic cells.
In vitro ...

In situ hybridization A technique in which cells are immobilized and their DNA is denatured and then hybridized to radioactive RNA probes; these hybrids are then detected by autoradiography.

In situ hybridization of a genome. This technique allows the discrimination between chromosomes originating from different species present within one nucleus.

Using in situ hybridization, researchers will first construct a rough map of the chromosome and then fill in the details with landmarks prepared by other methods.

Fluorescent in situ hybridization refers to using fluorescently labeled probe to hybridize to cytogenetic cell preparations.
In addition to standard preparations FISH can also be performed on: ...

Fluorescence in situ hybridization (FISH)
A physical mapping approach that uses fluorescein tags to detect hybridization of probes with metaphase chromosomes and with the less-condensed somatic interphase chromatin.
Related Terms:
Physical map ...

FISH -- florescent in situ hybridization: a technique for uniquely identifying whole chromosomes or parts of chromosomes using florescent tagged DNA.

FISH (fluorescence in situ hybridization) A physical mapping approach that uses fluorescein tags to detect hybridization of probes with metaphase chromosomes and with the less-condensed somatic interphase chromatin.

(fluorescence in situ hybridization): One of the more modern methods in cytogenetics, which uses fluorescence-labelled chromosome-specific DNA, probes to detect translocations, inversions, deletions, ...

One of those is called "in situ hybridization." One thing that I would've loved to have known back when I was initially studying the antennapedia mutant in fruit flies is where certain genes are expressed in the body.

FISH (fluorescence in situ hybridization): A mapping technique that uses fluorescent tags to identify specific locations of chromosomes.

In this newer technique, several different probes specific of one chromosome pair, carrying different amounts of a set of fluorescent dyes, are hybridized to the chromosomes in a technique known as fluorescent in situ hybridization (FISH).

Cells and tissues used and identification techniques: banding methods, high resolution banding and fluorescence in situ hybridization (FISH) and microdeletion
Chromosome Abnormalities: Autosomes and Sex Chromosomes ...

The method is a modification of fluorescence in situ hybridization (FISH) and is also called Zoo-FISH.

See also: Hybridization, Hybrid, Human, Chromosome, DNA