Lac repressor
From Wikipedia, the free encyclopedia
Jump to: navigation, search ...
Lac repressor
The lac repressor is an enzyme which stops the metabolism of lactose in bacteria as long as glucose is available.
Lac repressor The regulator protein that binds to the operator site of the lac operon and thereby inhibits expression of the structural genes of the operon; inhibition is relieved when the repressor protein binds allolactose, ...
The lac repressor represents only a tiny fraction of the proteins in the E. coli cell.
Link to a discussion of how it can nonetheless be isolated and purified.
The operon is the combination of the
operator and its associated
structural genes.
The plasmid contains a Lac repressor gene. Insertion of a DNA fragment inside the repressor gene disrupts its function, resulting in GFP expression. We currently have vector strains with Blue as well as Green Fluorescent Proteins.
The Oc is a mutation of the lactose operator that is no longer able to bind the lac I gene product which codes for the lac repressor.
 See also: DNA, Repressor, Protein, Trans, Expression
  
|
RSS
