Lambda phage
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Lambda phage
Enterobacteria phage λ (lambda phage) is a temperate phage that lives in E. coli. Once the phage is inside its host, it may integrate itself into the host's DNA.
Cosmids can be packaged in lambda phageparticles for infection into Escherichia coli; this permits cloning of larger DNA fragments (up to 45 kb) than can be introduced into bacterial hosts in plasmid vectors.
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Cloning vector ...
Cosmids replicate as plasmids in the host cell, but are inserted into the bacterium from lambda phage infectious particles, since transformation would be extremely inefficient with plasmids of this size.
Large, fluorescently stained restriction fragments of lambda phage DNA are sized by passing individual fragments through a focused, continuous- wave laser beam in an ultrasensitive flow cytometer at a rate of ~60 fragments per second.
This enables recombinant molecules to be packaged into lambda phage particles which are able to infect recipient bacteria. It is thus a very efficient way of creating libraries of relatively high molecular weight genomic DNA.
Cosmid
A cloning vector consisting of the phage lambda cos site inserted into a plasmid. Such vectors can be packaged into lambda phage or maintained as plasmids. Cosmids are often used to clone large DNA fragments (up to about 40 kilobases).
An E. Coli virus. Commonly used bacteriophases in biology labs are T4, M13 and lambda phages.
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 See also: DNA, Lambda, Phage, Genome, Chromosome
  
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