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Ligation

Biology LigaseLight reactions

Ligation
Enzyme catalyzed joining of two DNA fragments. The two DNA fragments must have compatible ends and at least one of the ends must have a 3' phosphate group.
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Ligation
The formation of a phosphodiester bond between two adjacent bases separated by a single-strand break. Catalyzed by DNA ligase.

Ligation-mediated PCR: This method uses small DNA linkers ligated to the DNA of interest and multiple primers annealing to the DNA linkers; it has been used for DNA sequencing, genome walking, and DNA footprinting. [22] ...

Ligation reactions Reactions that form bonds by using the energy of ATP hydrolysis.

tubal ligation A contraceptive procedure in women in which the oviducts are cut, preventing the ova from reaching the uterus.
tubal pregnancy Occurs when the morula remains in the oviduct and does not descend into the uterus.

Ligation
The process of joining two or more DNA fragments together.
Related Terms:
Deoxyribonucleic acid (DNA) ...

LIGATION - See DNA LIGASE.
LINEAR EPITOPE - An epitope formed by a series of amino acids which are adjacent to each other within the primary structure of the protein.

Ligation: The process of splicing two pieces of DNA together. In practice, a pool of DNA fragments are treated with ligase (see "Ligase") in the presence of ATP, and all possible splicing products are produced, ...

Unambiguous ligation requires that alternative complexes with perfect base pairing not be possible with the combination of hexamers used.

Sterilization
Roughly one-third of U.S. couples still in their reproductive years have chosen for one or the other to be sterilized.
Tubal Ligation ...

Ethics -- the study of fundamental principles which defines values and determines moral duty and obligation. Erythrocytes -- the hemoglobin-containing cell found in the blood of vertebrates.

LigationIn vitro reaction used to make the covalent phosphodiester linkage between two double stranded DNA ends.

Relating to duty or obligation; pertaining to those intentions and actions of which right and wrong, virtue and vice, are predicated, or to the rules by which such intentions and actions ought to be directed; relating to the practice, manners, ...

And I think that the case could be made that it is your obligation to allow the information about you to be used so your children and their children will have access to an equally good healthcare system in the future.

Physical prevention (most effective) include vasectomy and tubal ligation. Vasectomy: the vas deferens connecting the testes with the urethra is cut and sealed to prevent the transport of sperm.

Altruism is an ethical doctrine that holds that individuals have a moral obligation to help, serve, or benefit others, if necessary at the sacrifice of self interest.

Do individuals who test positive for HIV have an obligation to notify any of the following people?
People with whom they share living space
Current sexual partners
Former sexual partners
Doctors
Teachers
Coworkers ...

This should not be surprising; as discussed above, evolution as a problem-solving strategy is under no obligation to find the single best solution, merely one that is good enough.

See also: Trans, DNA, Sequence, Human, Gene

Biology LigaseLight reactions

 
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