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Melting

Biology MeiosisMelting temperature

Melting temperature
At the melting temperature half of the oligo will form duplex with complementary oligo at the same concentration. PCR annealing temperature is usually set a few degrees lower than the melting temperature. See also PCR Glossary.

 


Melting temperature The temperature at which secondary or higher structures of a biological molecule are lost; for a nucleic acid, the melting temperature is defined as the temperature at which half the helical structure is lost.

Melting point
α-D-glucose: 146°C
β-D-glucose: 150°C
Except where noted otherwise, data are given for
materials in their standard state
(at 25 °C, 100 kPa)
Infobox disclaimer and references ...

Tm: The melting point for a double-stranded nucleic acid. Technically, this is defined as the temperature at which 50% of the strands are in double-stranded form and 50% are single-stranded, i.e. midway in the melting curve.

latent heat of melting The amount of heat energy needed to melt a substance, that is, to change it from a solid to a liquid.

[More] (2) DNA: Separation of the two strands of the double helix (sometimes called "melting"). [More] Dendrite Dendritic cellsDendritic-cell vaccines DendrogramSee phylogenetic tree.

The choice of the length of the primers and their melting temperature depends on a number of considerations.

We assessed the effects of modifications on DNA melting, electrophoretic mobility and DNA-protein interaction for individual oligonucleotides and their small libraries.

DNA hybridization involves the "melting" of the DNA or separation of the two strands of the DNA molecule from two different species and allowing them to re anneal the speed and completeness of the association of DNA from different species is a ...

2. (Science: chemistry) An iron bar used for tapping a melting furnace. Lancet arch a large, voracious, deep-sea fish (Alepidosaurus ferox), having long, sharp, lancetlike teeth. The doctor, or surgeon fish.

Properties which affect a solvent based on the number of molecules of solute present: Melting point, boiling point and osmotic pressure.
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...

Glaciers would melt and sea levels would rise due to melting and expansion of warm water. Water evaporation would increase with increased rainfall along coasts and dry conditions inland.

-COOH group with double bonds in the carbohydrate chain
Low melting point / liquid at room temperature (oils)
Found in plants
Phospholipids ...

esker -- n. A ridge of glacial sediment deposited by a stream flowing in and under a melting glacier.
esophagus -- That portion of the gut which connects the pharynx to the stomach.

Separation of the two strands of a double-stranded DNA or RNA molecule by disrupting the hydrogen bonds that join the complementary bases from the two opposite strands, usually by heat or alkali. (Often called "melting".) ...

The Global Warming problem can lead to melting of the ice caps in Greenland and Antarctica, raising sea-level as much as 120 meters.

See also: Biology, Environment, Trans, Organ, DNA

Biology MeiosisMelting temperature

 
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