Nick

nick
(Science: molecular biology) A point in a double stranded dNA molecule where there is no phosphodiester bond between adjacent nucleotides of one strand typically through damage or enzyme action.

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Nick translation
A method which uses DNA polymerase I to first produce a nick in a DNA duplex, then degrade stretch of single-stranded DNA using its 5'-exonuclease while synthesizing a new strand in its place.

Nick translation. A procedure for making a DNA probe in which a DNA fragment is treated with DNase to produce single-stranded nicks, followed by incorporation of radioactive nucleotides from the nicked sites by DNA polymerase I.

Nick translation
Method used to introduce 32p into a DNA probe so that the probe can be detected.
Related Terms:
Deoxyribonucleic acid (DNA) ...

NICK TRANSLATION - A method for introducing labeled nucleotides into a double-stranded DNA molecule which involves making small nicks in one strand with DNase, and then repairing with DNA polymerase I.

Nick translation: A method for incorporating radioactive isotopes (typically 32P) into a piece of DNA. The DNA is randomly nicked by DNase I, and then starting from those nicks DNA polymerase I digests and then replaces a stretch of DNA.

When conjugation is initiated, via a mating signal, a relaxase enzyme creates a nick in one plasmid DNA strand at the origin of transfer, or oriT.

If the F-plasmid is free, a nick is produced in one of the circular DNA strands of the plasmid. Then, the single-stranded DNA of the nicked strand is inserted into the recipiane bacterium (5'-end first).

Reaction conditions have been defined where purified f1 gene 2 protein efficiently introduces a strand-specific single nick in the f1 origin sequence with very little rejoining.

And amongst the red cells is an array of specialized cells - neutrophils, platelets, monocytes, lymphocytes - ready to spring into action at the slightest nick of the skin.
To see how immune cells become involved when we get a splinter or a scratch...

Due to mis-use by early farmers and a very long dry period of time called a drought, the North American grasslands became a giant dusty mess in the 1930s. During this time, this area was nick named the dust bowl.

See also: Trans, DNA, Strand, Biology, Protein