Nick translation
A method which uses DNA polymerase I to first produce a nick in a DNA duplex, then degrade stretch of single-stranded DNA using its 5'-exonuclease while synthesizing a new strand in its place.
Nick translation. A procedure for making a DNA probe in which a DNA fragment is treated with DNase to produce single-stranded nicks, followed by incorporation of radioactive nucleotides from the nicked sites by DNA polymerase I.
Nick translation
Method used to introduce 32p into a DNA probe so that the probe can be detected.
Related Terms:
Deoxyribonucleic acid (DNA) ...
NICK TRANSLATION - A method for introducing labeled nucleotides into a double-stranded DNA molecule which involves making small nicks in one strand with DNase, and then repairing with DNA polymerase I.
Nick translation: A method for incorporating radioactive isotopes (typically 32P) into a piece of DNA. The DNA is randomly nicked by DNase I, and then starting from those nicks DNA polymerase I digests and then replaces a stretch of DNA.
 See also: DNA, Nucleotide, Strand, Hybrid, Hybridization
  
|
RSS
