Northern blotting

Northern blotting. See Northern hybridization.
Northern hybridization. (Northern blotting).

Northern blotting Analogous to Southern blotting, a technique in which a mixture of RNA fragments is separated by electrophoresis, transferred to a nitrocellulose sheet, ...

[edit] Northern blotting
Main article: Northern blot
The Northern blot is used to study the expression patterns a specific type of RNA molecule as relative comparison among of a set of different samples of RNA.

Northern blotting
Method by which RNA is analyzed. RNA is separated by size, ...

Similar to Southern or Northern blotting (see "Blotting"), except that (1) a protein mixture is electrophoresed in an acrylamide gel, and (2) the "probe" is an antibody which recognizes the protein of interest, ...

of annealing one strand of a polynucleotide (DNA or RNA), the probe, to a strand of complementary sequence (again either DNA or RNA), the target. Also, the process of forming a hybrid. Used in many techniques such as Southern and Northern Blotting, ...

See also: Blotting, DNA, Hybrid, Protein, Probe