Northern hybridization

Northern hybridization. (Northern blotting). A procedure in which RNA fragments are transferred from an agarose gel to a nitrocellulose filter, where the RNA is then hybridized to a radioactive probe. (See Hybridization.) ...

Northern hybridization
... ... the quantification of DIG-labeled RNA and as a hybridization . control ... Note: Hybridization temperature for Northern Blots with DIG Easy hyb is 68 °C (for ... Northern Hybridization Solution. AB10055. DEPC treated.

(See Northern hybridization, Southern hybridization.) Hybridoma. A hybrid cell, composed of a B Iymphocyte fused to a tumor cell, which grows indefinitely in tissue culture and is selected for the secretion of a specific antibody of interest.

Data concerning physical map assembly, genomic sequencing, Northern hybridization, tumor mapping, public database searches and extensive sequence annotation efforts including graphical maps and detailed data on clones, loci, exons, ...

This use of PCR augments many methods, such as generating hybridization probes for Southern or northern hybridization and DNA cloning, which require larger amounts of DNA, representing a specific DNA region.

bases to form double-stranded molecules; this process is the basis for molecular biological techniques in which a labeled probe sequence is used to detect another identical or similar sequence (e.g., Southern hybridization, Northern hybridization).

See also: Biology, Hybridization, Complement, Strand, Nucleic acid