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An in situ methodology employing solution conditions has been developed for binding oligodeoxyribonucleotide 'third-strands' to chromosomal DNA targets in non-denatured protein-depleted metaphase spreads and interphase nuclei.
"Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides". Gene 164: 49-53. PMID 7590320.
^ Innis MA, Myambo KB, Gelfand DH, Brow MA. (1988).
 See also: Mutation, Nucleotide, PCR, Colon, Gene
  
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