Oligonucleotide
A short sequence of polynucleotide. Oligonucleotides with a defined sequence can synthesized in vitro for use for a variety of techniques, including PCR, DNA sequence analysis, hybridization, and site-directed mutagenesis.
Oligonucleotide primers. Libraries of oligonucleotide primers 9 or 10 nucleotides in length could greatly reduce the cost and effort of high-volume sequencing. Currently, the feasibility of this strategy is being tested.
oligonucleotide - piece of single-stranded nucleic acid, short stretch of nucleotides.
Oligonucleotide A short (2 - 50 nucleotides) sequence of (usually single stranded) DNA which has been chemically synthesised for a specific experimental purpose.
Oligonucleotide. A DNA polymer composed of only a few nucleotides. (See Nucleotide.) ...
[edit] Oligonucleotides
The use of synthetic oligonucleotides in gene therapy is to inactivate the genes involved in the disease process. There are several methods by which this is achieved.
Oligonucleotide
A short length of nucleic acid composed of between two to approximately 10 nucleotides.
OLIGONUCLEOTIDE - See OLIGODEOXYRIBONUCLEOTIDE.
ONCOGENE - One of a number of genes believed to be associated with the malignant transformation of cells; ...
A short oligonucleotide complementary to target DNA and acts as the leader for DNA extension. Also refers to short oligonucleotide used to primer reverse transcription of RNA to DNA in reverse transcription reactions. See PCR Glossary.
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RT ...
Primer: A small oligonucleotide (anywhere from 6 to 50 nt long) used to prime DNA synthesis.
A short, double-stranded oligonucleotide containing a restriction endonuclease recognition site, which is ligated to the ends of a DNA fragment. Liposomes. Membrane-bound vesicles constructed in the laboratory to transport biological molecules.
In practice, when synthesising an oligonucleotide primer or probe based on this information a mixture of all the possible sequences is made, and referred to as a degenerate oligonucleotide.
Oceanic zone Okazaki fragment OKT3[More] Olfaction, the sense of smell OligonucleotideA short polymer of, for example, 20 or so deoxyribonucleotides or ribonucleotides; thus a fragment of DNA or RNA. Ommatidia Oncogenes OncogenicCancer causing.
Khorana was also the first to synthesize oligonucleotides , that is, strings of nucleotides. These custom designed pieces of artificial genes are widely used in biology labs for sequencing, cloning and engineering new plants and animals.Dr.
These primers are short oligonucleotides chosen by the experimenter and added to the DNA mixture at the beginning. They flank the region to be amplified. One binds to the + strand and the other binds to the - strand.
Two oligonucleotide primers (oligos) are constructed to flank a region of interest. One oligo will be complementary to a region on one strand of DNA, and the other oligo will be complementary to a region downstream on the homologous strand.
Arbitrary primer
A short oligonucleotide primer used in certain PCR methods to initiate DNA synthesis at random locations on the target DNA.
The technology utilizes DNA fragments that are ligated to complementary adaptor oligonucleotide and subsequent rounds of PCR amplification using primers complementary to the adaptor sequences.
- A synthetic single-stranded non self-complementary oligonucleotide used in conjunction with alinker to add cohesive ends to DNA molecules
Additive genes ...
A machine which automatically makes short, artificial polynucleotides or oligonucleotides with any desired sequence of nucleotide bases.
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...
Which of the following type(s) of polymorphism are commonly detected by using the polymerase chain reaction (PCR) and specific oligonucleotide primers?
A.
RFLPs, "restriction fragment length polymorphisms" ...
sequence used in the polymerase chain reaction (PCR) technique. In the PCR method, a pair of primers is used to hybridize with the sample DNA and define the region of the DNA that will be amplified. Primers are also referred to as oligonucleotides.
Depurination is also a well-known side reaction in DNA synthesis and in isolation of DNA or RNA samples from gels, when the oligonucleotide is subjected to weak acid conditions. During synthetic deprotection of trityl plus some . . . a test ...
VNTR -- variable number tandem repeats; any gene whose alleles contain different numbers of tandemly repeated oligonucleotide sequences. Vector -- a self-replicating DNA molecule that transfers a DNA segment between host cells.
 See also: DNA, Sequence, Nucleotide, Genome, Human
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