Overhang
Sticky ends generated by restriction digestion of double-stranded DNA.
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[edit] Overhangs and sticky ends
Non-blunt ends are created by various overhangs. An overhang is a stretch of unpaired nucleotides in the end of a DNA molecule.
The overhangs thus produced can still hybridize ("anneal") with each other, even if they came from different parent DNA molecules, and the enzyme ligase will then covalently link the strands.
Homopolymer tailing Extension of the 3' ends of a piece of double-stranded DNA using the enzyme terminal transferase and a single deoxynucleoside triphosphate as substrate, resulting in a 3' overhang composed of a single base repeat.
The fragment ends are then blunted by treatment with Mung Bean nuclease and T4 DNA polymerase, and are ligated to the blunt-end side of an adapter which has a 3' overhang (ACAC) at the other end.
It begins at the vertical border by a smooth, triangular area over which the tendon of insertion of the lower part of the Trapezius glides, and, gradually becoming more elevated, ends in the acromion, which overhangs the shoulder-joint.
Because recognition sequences differ between restriction enzymes, the length and the exact sequence of a sticky-end "overhang", as well as whether it is the 5' or the 3' strand that overhangs, depends on which enzyme produced it.
The ends of the cut have an overhanging piece of single-stranded DNA.
These are called "sticky ends" because they are able to base pair with any DNA molecule containing the complementary sticky end.
2. Jutting over; projecting; overhanging. A vapor sometime like a . . . Pendent rock.
Origin: L. Pendens, -entis, p.pr. Of pendere to hang, to be suspended. Cf. Pendant.
- DNA termini without overhanging 3' or 5' ends. Also knows as flush ends ...
Cohesive end: Also known as sticky end. Overhanging ends of a double-stranded DNA molecule that are capable of hybridizing with complementary ends.
The end of a tRNA molecule to which an amino acid becomes bound. It contains both the 5' and 3' ends of the tRNA. The 3'-terminal sequence of cytidine-cytidine-adenosine (CCA) overhangs the end, ...
 See also: Base, DNA, Human, Restriction, Biology
  
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