Overlapping clones

Overlapping clones: See genomic library.
P
P1-derived artificial chromosome (PAC): A vector used to clone DNA fragments (100- to 300-kb insert size; average, 150 kb) in Escherichia coli cells. Based on bacteriophage (a virus) P1 genome.

Overlapping clones
See genomic library.
Related Terms:
Genomic library
A collection of clones made from a set of randomly generated overlapping DNA fragments representing the entire genome of an organism. Compare library, arrayed library.

Working from a flanking DNA marker, overlapping clones are successively identified that span a chromosomal region of interest. (See Chromosome.) Cistron. A DNA sequence that codes for a specific polypeptide; a gene. See DNA, Gene. Clone.

A genetic map depicting the relative order of a linked (contiguous) library of small overlapping clones representing a complete chromosomal segment.

Chromosome walking. Working from a flanking DNA marker, overlapping clones are successively identified that span a chromosomal region of interest. (See Chromosome.) ...

A map depicting the relative order of a linked library of overlapping clones representing a complete chromosomal segment.
Cosmid ...

Chromosome walking A method of making a detailed map of a chromosome by isolating successive overlapping clones. Usually each clone is used as a probe to isolate the next.
Clone ...

Oncogene A gene, one or more forms of which is associated with cancer. Many oncogenes are involved, directly or indirectly, in controlling the rate of cell growth.
Overlapping clones See genomic library.
Ovum Female egg ...

A restriction fragment isolated from the end of the positive clones is used to reprobe the genomic library for overlapping clones. This process is repeated several times to walk across the chromosome and reach the gene of interest.

See also: Clones, Clone, DNA, Genome, Chromosome