Polymerase chain reaction

Polymerase Chain Reaction (PCR) is a molecular biological technique for amplifying (creating multiple copies of) DNA without using a living organism, such as E. coli or yeast.

Polymerase chain reaction
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The Polymerase Chain Reaction (PCR): Cloning DNA in the Test Tube
The polymerase chain reaction is a technique for quickly "cloning" a particular piece of DNA in the test tube (rather than in living cells like E. coli).

Polymerase Chain Reaction (PCR)
Polymerase chain reaction (PCR) is a laboratory technique used to amplify DNA sequences. The method involves using short DNA sequences called primers to select the portion of the genome to be amplified.

Polymerase Chain Reaction (PCR)
The technique of PCR permits the selective amplification of a specific segment of DNA or RNA. Some sequence information must be known prior to PCR so that primers flanking the segment of interest can be synthesized.

Polymerase chain reaction (PCR): A "biological copy machine": a method for making many copies of a specific DNA base sequence.
Polypeptide: A molecule made up of a stri ng of amino acids. A protein is an example of a polypeptide.

Polymerase chain reaction
See PCR.
Polysome
An mRNA molecule that is in the process of being translated by multiple ribosomes simultaneously.

polymerase chain reaction (PCR)
A technique for amplifying DNA in vitro by incubating with special primers, DNA polymerase molecules and nucleotides.
polymorphic ...

polymerase chain reaction (PCR) A method of amplifying or copying DNA fragments that is faster than cloning.

Polymerase chain reaction (PCR) A technique by which a relatively small piece of DNA of known sequence can be amplified (often from a complex mixture) by successive cycles of strand separation followed by DNA synthesis (using a DNA polymerase ...

polymerase chain reaction - powerful DNA synthesis and amplification technique allowing the amplification of a specific sequence from among many others; thoeoretically PCR can be used to detect as little as one specific DNA molecule.

polymerase chain reaction (PCR). A procedure that en- zymatically amplifies a DNA polymerase. (See Polymerase.)
Polypeptide (protein). A polymer composed of multiple amino acid units linked by peptide bonds.

Polymerase Chain Reaction (PCR)
The polymerase chain reaction can be used to make many copies of small pieces of DNA.

Polymerase chain reaction (PCR)
A method for amplifying a DNA base sequence using a heat-stable polymerase and two 20-base primers, ...

Polymerase chain reaction (PCR)
A method, developed by Kary Banks Mullis 1983, for amplifying a DNA base sequence using a heat-stable polymerase and two 20-base primers, ...

Polymerase chain reaction (PCR) is one technique for studying organisms that cannot be grown in the laboratory.

Polymerase chain reaction (PCR)
- A technique for quickly making many copies of a specific segment of DNA.
Polymorphism ...

polymerase chain reaction (PCR) - technique for amplifying specific regions of DNA by multiple cycles of DNA polymerization, each followed by a brief heat treatment to separate complementary strands.

Polymerase chain reaction: A technique for replicating a specific piece of DNA in-vitro , even in the presence of excess non-specific DNA. Primers are added (which initiate the copying of each strand) along with nucleotides and Taq polymerase.

polymerase chain reaction (PCR). A technique for preparing large quantities of DNA from tiny samples, making it easy to clone a specific gene as long as part of the sequence of the gene is known.

Inverse polymerase chain reaction (IPCR)
A technique to amplify genomic DANN flanking the insertion site of a transposon or T-DNA construct.

PCR Polymerase chain reaction. An in vitro technique to produce many copies of a specific section of DNA sequence. PCR is normally used to amplify sections up to ~2kbp in length, although routine PCR of sections up to 20kbp is becoming possible.

PCR - See POLYMERASE CHAIN REACTION
PEPTIDE - A chain formed by two or more amino acids linked through peptide bonds: dipeptide = two amino acids, oligopeptide = small number of amino acids, etc.

PCR See polymerase chain reaction.
Penetrance An "ll or none" reference to clinical expression of a mutant gene.
Phage A virus for which the natural host is a bacterial cell.

Introduction to polymerase chain reaction (PCR)
The universe of exons
DNA sequenced from 17-20 MY old magnolia
Introns of ancient origins ...

To increase the number of copies of a DNA sequence, in vivo by inserting into a cloning vector that replicates within a host cell, or in vitro by polymerase chain reaction (PCR). Anaerobe. An organism that grows in the absence of oxygen. See Aerobe.

PCR -- polymerase chain reaction; a technique for copying the complementary strands of a target DNA molecule simultaneously for a series of cycles until the desired amount is obtained.

PCR See Polymerase chain reaction (PCR)
PFGE See Pulsed-Field Gel Electrophoresis (PFGE)
Phenotype
Phosphatase
Polyacrylamide Gel Electrophoresis (PAGE)
Polymerase Chain Reaction (PCR)
Polymerase chain reaction (PCR)
Polymorphism ...

Polymerase chain reaction (← links)
Blot western (← links)
Saccharomyces cerevisiae (← links)
Dominant lethal trait (← links)
Arabidopsis thaliana (← links)
Axonal transport (← links)
Receptors cholinergic (← links) ...

We will assume that you have a basic understanding of the Polymerase Chain Reaction (PCR), and gel electrophoresis, especially as applied to DNA sequence analysis.

Reverse Transcriptase Polymerase Chain Reaction
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The cDNA is then amplified using standard PCR protocols.
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z ...

quantitative PCR
quantitative RT-PCR
quantitative trait
quantitative trait locus
quantitative variable
quantitative variation
Quantitative-fluorescent polymerase chain reaction
quaternary structure
quiescent ...

See also: Polymerase, Action, DNA, Sequence, Trans