Primase
DNA polymerase cannot initiate a new strand, it can only elongate a strand that is already present. Synthesis of new DNA therefore cannot begin until a short strand of nucleotides is added. This short strand is called a primer.
Primase
An enzyme that synthesizes ribonucleotide primers for lagging strand DNA synthesis of Okazaki fragments. Polymerizes ribonucleotide triphosphates in the 5' to 3' direction.
Primase A specialized RNA polymerase that synthesizes the RNA primers for DNA synthesis.
Primer In the elongation of polymers, the initial segment of the polymer that is to be extended; elongation depends on the primer.
The complex of T7 DNA polymerase and T7 helicase/primase synthesize DNA with high efficiency. We have been optimizing reactions carried out by these enzymes in combination with other T7 replication proteins.
On the lagging strand, primase "reads" the DNA and adds RNA to it in short bursts. Pol III lengthens the bursts, forming Okazaki fragments.
Pre-replication complex: Helicase (dnaA, dnaB, T7) - Primase (dnaG) - DNA polymerase III holoenzyme (dnaQ)
DNA ligase - Telomerase - Topoisomerase
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primosome - unit formed on the lagging strand comprised of a primase molecule and helicase
promoter - nucleotide sequence in DNA to which RNA polymerase binds to begin transcription ...
The DNA-replicating structure at the replication fork consisting of two DNA polymerase III enzymes and a primosome (primase and DNA helicase).
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Primase (← links)
Trna (← links)
Signal recognition particle (← links)
Ribonuclease (← links)
Respiratory syncytial virus (← links)
Leukocyte interferon (← links)
Nucleolus (← links)
Fibroblast interferon (← links) ...
 See also: Replication, DNA, Enzyme, Strand, Nucleotide
  
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